Gross Structure of Antibodies

Question 1

What is another name of antibodies?

Answer 1

Immunoglobulins or IGs

Question 2

What is the B cell receptor?

Answer 2

A membrane bound form of an antibody with the same structural features and same antigen specificity

Question 3

What are the 5 classes or isotypes of antibodies?

Answer 3

IgA, IgD, IgE, IgG, and IgM

Question 4

Epitope

Answer 4

A small specific area on a pathogen which is recognized by an antibody

Question 5

The specificity of an antibody is determined by which region?

Answer 5

The variable region

Question 6

The constant region determine what of an antibody?

Answer 6

Function

Question 7

The constant region of an antibody determines what?

Answer 7

Function

Question 8

What four polypeptide chains makeup an antibody molecule?

Answer 8

2 H chains and 2 L chains

Question 9

What is the V region?

Answer 9

The N-terminus of each chain, known as the variable region

Question 10

What is the C region of an antibody?

Answer 10

The rest of the H or L chain that differs only in determining isotype

Question 11

What type of polymer is Igm?

Answer 11

Pentamer or hexamer

Question 12

What type of polymer is IgA?

Answer 12

Dimer or monomer

Question 13

What bonds hold together the H and L chains?

Answer 13

Disulfide

Question 14

Which chains do not interact?

Answer 14

The two L chains

Question 15

How many disulfide bonds hold the H chains together?

Answer 15

2

Question 16

How many bonds hold an H chain and L chain together?

Answer 16

1

Question 17

What are the two types of L chains?

Answer 17

Kappa and Lambda

Question 18

How many amino acids are found in a domain?

Answer 18

About 110

Question 19

How many domains does an H chain have?

Answer 19

3

Question 20

How many domains does and L chain have?

Answer 20

1

Question 21

How many domains are found in IgG, IgA, and IgD?

Answer 21

3

Question 22

How many domains are found in IgM and IgE?

Answer 22

4

Question 23

Which isotypes do not have a hinge?

Answer 23

IgE and IgM

Question 24

What is the single effector function of B cells?

Answer 24

To produce antibodies

Question 25

Which isotypes have 4 Ch domains?

Answer 25

IgM and IgE

Question 26

Which isotypes have 3 constant domains?

Answer 26

IgA, IgD, and IgG

Question 27

Domains have about how many amino acids?

Answer 27

110

Question 28

Which portion of the antibody determines its function?

Answer 28

The constant region

Question 29

What is the ratio in weight of heavy chain to light chain?

Answer 29

2:1

Question 30

What is fab?

Answer 30

The fraction antigen binding site

Question 31

What is Fc?

Answer 31

Fraction crystallizable

Question 32

Which isotypes have the hinge present?

Answer 32

IgG, IgA, and IgD

Question 33

Which enzyme must antibodies be treated with to make Fab and Fc?

Answer 33

Papain

Question 34

True or False. Fab can retain the ability to mind antigen.

Answer 34

True

Question 35

Which region contains the hinge? Fab or Fc.

Answer 35

Fc

Question 36

What is the function of the Fc region?

Answer 36

Opsonization and deliver the antibody to specific anatomical sights

Question 37

Which enzyme is used to make F(ab')2?

Answer 37

Pepsin

Question 38

What pieces are found in F(ab')2?

Answer 38

Both Fab regions and the hinge

Question 39

Where is the hinge found?

Answer 39

Between Ch1 and Ch2

Question 40

Which residues primarily makeup the hinge region?

Answer 40

Pro, Ser, and Thr

Question 41

How many epitopes does the hinge allow Fab to bind?

Answer 41

2

Question 42

Which isotypes are associated with the J chain?

Answer 42

IgA and IgM

Question 43

Which form of IgM does not have a J chain?

Answer 43

Hexameric IgM

Question 44

How many J chains are found per antibody polymer?

Answer 44

1

Question 45

What is transcytosis?

Answer 45

Transport of antibodies from the basal side of the epithelium to the mucosal surface

Question 46

Papain digestion results in how many pieces?

Answer 46

3

Question 47

Pepsin digestion results in how many pieces?

Answer 47

1 the F(ab')2