Haemophilus influenzae

Question 1

morphology

Answer 1

capsulated gram negative coccobacilli pleomorphic rods;

Question 2

capsulated or non capsulated

Answer 2

can be both

Question 3

Virulence

Answer 3

Capsule - polyribitol phosphate which is found in cell wall !!component of the HIB vaccine

Polysaccharide B

IgA protease - evasion

X(hemin) and V(NAD) - growth

PAMP

Question 4

What makes noncapsulated aka non-typeable Haemophilus influenzae different?

Answer 4

NTHi strain can not be neutralized by HIB vaccine

Question 5

capsulated Haemophilus influenzae attacks who?

Answer 5

B cell deficient/ IgA deficient

Question 6

Nontypeable Haemophil influenzae causes what?

Answer 6

sinusitis, otitis, pneumonia, COPD patients

Question 7

Lab diagnosis

Answer 7

Chocolate agar - X&V growth factors assist latex agglutination test checking for capsule antigen

Gram stain will be positive (organ and red demonstrating neutrophils)

Question 8

Vaccine

Answer 8

Hib/PRP conjugate vaccine; t-dependent antigenic stimulation