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hanging drop Flashcards

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1
Q

deamination of acetamide results in a ______ color

A

(+) blue
(-) green (no color change)

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2
Q

Acetamide Utilization Test
POS Control
NEG Control

P. aeruginosa
E. faecalis
N. gonorrhea
E.coli

A

POS Control: P. aeruginosa
NEG Control E.coli

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3
Q

Differentiate organism based on their ability to use acetate as a sole source of carbon. Generally differentiates Shigella from E. coli

A

Acetate utilization

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4
Q

POS result for acetate utilization
NEG result

A

(+) blue
(-) no color change or green

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5
Q

Acetate Utilization
POS control
NEG control

Shigella sonnei
S. pneumoniae
E. coli
B. mallei

A

POS control: E. coli
NEG control: Shigella sonnei

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6
Q

Test is used for the presumptive identification of Strep Grp A (S. pyogenes) from other b-hemolytic strep

Staphyloccoccus from Micrococcus

A

Bacitracin (0.04 unit Taxo A)
POS control: S. pyogenes, Microccus luteus
NEG control: S. agalactiae, S aureus

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7
Q

This test differentiates enterococci and group D streptococci from non-group D viridans strep

acetate utilization
bile solubility test
butyrate disk
bile esculin test

A

Bile esculin test

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8
Q

Positive result for bile esculin agar

A

(+) blackening of the slant or medium
brown black precipitate

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9
Q

Bile Esculin Agar
POS control
NEG control

S. agalactiae
E. coli
S. pyogenes
E. faecalis

A

POS control: E. faecalis
NEG control: E. coli
S. pyogenes

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10
Q

differentiates streptococcus pneumoniae from alpha hemolytic streptococci

bile esculin agar
butyrate disk
bile solubility test

A

Bile solubility Test
(+) lysed colonies

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11
Q

Bile solubility test
POS control
NEG control

S. agalactiae
E. coli
S. pneumoniae
E. faecalis

A

POS control: S. pneumoniae
NEG control: E. faecalis

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12
Q

Rapid test to detect enzyme butyrate esterase to aid identification of M. catarrhalis

A

Butyrate Disk
NEG control = N. gonorrhea
POS = M. catarhhalis

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13
Q

POS result for butyrate disk

A

blue w/in 5 mins
indigo to violet

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14
Q

Used to differentiate Grp B strep (S. agalactiae from other streptococcal spp.

L. monocytogenes is also positive

A

CAMP Test

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15
Q

CAMP Test
POS Control
NEG control

S. aureus
S. agalactiae
S. pyogenes
E. coli

A

POS Control: S. agalactiae
NEG control: S. pyogenes

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16
Q

differentiates microccal and staphylococcal species from streptococcal spp. (neg)

A

catalase
(+) bubbles, hydrogen peroxide

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17
Q

Catalase
POS control
NEG control

S. aureus
S. agalactiae
S. pyogenes
E. coli

A

POS control: S. aureus
NEG control: S. pyogenes

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18
Q

Test used to isolate and purify p. aeruginosa

A

cetrimide
(+) growth

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19
Q

Cetrimide
POS control
NEG control

E. faecalis
P. aeruginosa
Providencia
E. coli

A

POS control: P. aeruginosa
NEG control: E. coli

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20
Q

Indicator of citrate test

A

bromthymol blue
(+) blue / growth

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21
Q

Citrate test
POS control
NEG control

E. aerogines
P. aeruginosa
K. pneumoniae
E. coli

A

POS control: E. aerogines, K. pneumoniae
NEG control: E. coli

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22
Q

Coagulase Test
POS control
NEG control

S. epidermidis
E. coli
S. aureus

A

POS control: S. aureus
NEG control: S. epidermidis

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23
Q

Differentiate decarboxylase producing enterbacteriaceae from other gram neg rods

A

decarboxylase test (moeller’s)
LOA
(+) purple
(-) yellow or no color change

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24
Q

POS Control for LOA
Lysine
Ornithine
Arginine

E. aerogenes
K. pneumoniae
E. cloaccae

A

Lysine: K. pneumoniae
Ornithine: E. aerogenes
Arginine: E. cloaccae

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25
NEG Control for LOA Lysine Ornithine Arginine E. coli P. vulgaris C. freundii
Lysine: C. freundii Ornithine: P. vulgaris Arginine: E. coli
26
DNA hydrolysis pos result
colorless around the test organism (-) medium remains green = no lysis of dna
27
DNA Hydrolysis POS control NEG control S. epidermidis E. coli S. aureus
POS control: S. aureus NEG control: E. coli
28
Test for enterbacteriaceae in which a positive result is black precipitate with no fluorescence
Esculin hydrolysis (-) No blackening and fluorescence
29
Esculin Hydrolysis POS control NEG control S. epidermidis E. faecalis E. coli S. aureus
POS control: E. faecalis NEG control: E. coli
30
Peptone medium with andrade's indicator is used to differentiate: ____________ BHI with bromcresol purple indicator is used to differentiate _____________ a. streptococci and enterococci b. enteric and coryneforms
Peptone medium with andrade's indicator is used to differentiate: B BHI with bromcresol purple indicator is used to differentiate: A a. streptococci and enterococci b. enteric and coryneforms
31
Peptone medium with andrade's indicator POS result NEG result
(+) pink with or without gas (-) no change in color medium (straw colored)
32
Andrade's indicator POS w/ gas POS w/o gas E. coli S. sonnei S. flexneri
POS w/ gas: E. coli POS w/o gas: S. flexneri
33
BHI with bromcresol purple POS result NEG result
POS result: yellow NEG result: no color change (purple)
34
BHI with bromcresol purple PC NC E. coli M. osteonsis S. flexneri
PC with gas: E. coli NC: Moraxella osteonsis
35
production of gelatinases capable of hydrolizing gelatin presumptive for staph, entero and some gram pos bacilli
Gelatin Hydrolysis (+) partial or total liquefaction within 4hrs at 4degrees (-) complete solidification at 4C
36
Gelatin hydrolysis PC NC E. coli B. subtilis B. cepacia
PC: B subtilis NC: E. coli
37
growth at 42C
Pseudomonas (PC) P. fluorescens (NC)
38
Hippurate Test POS result NEG result violet deep purple colorless to yellow pink colorless to purple
POS result: deep purple NEG result: colorless or yellow pink
39
Test used to identify organisms that produce tryptophanase
Indole
40
Indole POS result NEG result
POS result: pink to wine colored ring NEG result: no color change
41
Indole tests reagents
Kovac or Ehrlich
42
Hippurate Test PC NC S. agalactiae S. aureus S. maltophilia S. pyogenes
PC: S. agalactiae NC: S. pyogenes
43
Indole PC for Kovacs: PC for ehrlich: E. coli H. aegypticus H. influenzae E. tarda
PC for Kovacs: E. coli PC for ehrlich: H. influenzae
44
test that identifies catalase negative gram positive cocci using the cinnamaldehyde reagent Litmus Milk LAP Hippurate Andrade's
LAP (+) red color (leucine aminopeptidase)
45
LAP Test PC NC E. coli E. faecalis A. virdans
PC: E. faecalis NC: Aerococcus viridans
46
Litmus Milk Medium fermentation - gas production A. P. aeruginosa B. L. acidophillus C. C perfringens
C
47
Litmus Milk Medium Acid - clot formation A. P. aeruginosa B. L. acidophillus C. C perfringens
B
48
Litmus Milk Medium Peptonization - clearing A. P. aeruginosa B. L. acidophillus C. C perfringens
A
49
bright red color indicative of mixed acid fermentation
MR
50
red color indicative of acetoin production
VP
51
negative result for MRVP
yellow
52
MR POS, VP NEG control MR NEG, VP POS control E. coli S. sonnei E. aerogenes K. pneumoniae
MR POS, VP NEG control = E. coli MR NEG, VP POS control = E. aerogenes
53
This test differentiates gram pos. cat pos cocci oxidase microdase
Microdase
54
Positive result for microdase test
blue to purple blue color
55
Microdase Test PC NC S. aureus E. coli M. luteus
PC: M. luteus NC: S. aureus
56
Semisolid Agar deep - for motility POS result NEG result
POS result: organisms spread from site of inoculation NEG result: non-motile, remain at site of inoculation
57
PC for hanging drop and semisolid agar deep
E. coli
58
Used to determine if organism forms gas during glucose fermentation. Some lactobacillus and leuconostoc produce gas
MRS broth (+) gas production
59
MRS broth Growth, gas production: Growth, no gas:
Growth, gas production: leuconostoc Growth, no gas: lactobacillus
60
Identifies various genera of enterobateriaceae and verotoxin producing E. coli
MUG Test (+) electric blue fluorescence
61
MUG Test PC NC E. faecalis E. coli K. oxytica K. pneumoniae
PC: E. coli NC: K. pneumoniae
62
Determine whether an organism can reduce nitrite to gaseous nitrogen
Nitrite Reduction (+) no color change within 2 mins (-) red
63
Nitrite Reduction PC NC P. vulgaris P. mirabilis A. baumanii E. coli
PC: P. mirabilis NC: A. baumanii
64
Tests whether an organism can produce B-galactosidase
ONPG
65
Positive Result for ONPG blue green yellow pink
yellow (-) colorless
66
ONPG PC NC S. pneumoniae S. sonnei S. typhimurium
PC: S. sonnei NC: S. typhimurium
67
Positive result for optochin (zoi)
14mm or greater ZOI
68
Optochin PC NC S. pneumoniae S. sonnei S. pyogenes S. typhimurium
PC: S. pneumoniae NC: S. pyogenes
69
Positive result of oxidase test
dark purple color within 10 secs
70
Oxidase Test PC NC S. pyogenes S. aeruginosa S. typhimurium E. coli
PC: S. aeruginosa NC: E. coli
71
Reagent of Phenyalalanine Deaminase
ferric chloride
72
Phenyalanine Deaminase PC NC P. mirabilis P. vulgaris E. coli
PC: P. mirabilis NC: E. coli
73
Test that differentiates group A streptococci and enterococci by the presence of L-pyrolidonyl arylamidase
PYR test (+) bright red color, cinamaldehyde reagent
74
PYR Test PC (2) S. pyogenes E. coli E. faecalis S. aureus
S. pyogenes E. faecalis
75
Differentiates E. faecalis (pos) and E. faecium (negative) PYR Pyruvate broth Spot Indole
Pyruvate broth
76
Positive result for pyruvate Blue to green Green to yellow Yellow to blue
green to yellow
77
PC control for pyruvate broth and salt tolerance
E. faecalis
78
NC for salt tolerance
S. bovis
79
Positive result for salt tolerane
turbidity purple to yellow
80
Determine the presence of the enzyme tryptophanase
Spot indole (+) blue
81
PC for spot indole
E. coli
82
Proteus may be presumptively identified by the ability to hydrolyze ______
urea
83
Urea Hyrdrolysis Christensen's method POS result NEG result
POS result: light orange to magenta NEG result: no color change
84
Urea Hydrolysis PC NC P. vulgaris K. pneumoniae E. coli
PC: P. vulgaris Weakly Pos: K. pneumoniae NC: E. coli

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