Hemapoiesis Review

Question 1

Give the time period for liver phase.

Answer 1

1-9 months

Question 2

Name two adult loci for blood development

Answer 2

Myeloid tissue in bone, lymphoid tissue

Question 3

What is the characteristic shape of megakaryocyte?

Answer 3

Lobed, polyploidy nucleus (32-64N)

Question 4

Thromopoiesis
Give 5 stage of developmental sequence.
Where does it reside permanently? (it = megakaryocytes)
(nucleus)

Answer 4

MEP -> CFU-Meg -> Megakaryoblast -> Megakaryocyte -> Platelets
Bone marrow

Question 5

Give the time period for adult phase.

Answer 5

4+ months

Question 6

Give the time period for spleen phase.

Answer 6

3-6.5 months

Question 7

What cells do megakaryocytes give rise to?

Answer 7

platelets

Question 8

What characteristic feature do you see on megakaryocytes’ cytoplasm?

Answer 8

Demarcation channels

Question 9

Give the time period for Yolk-Sac phase.

Answer 9

Conception – 3 months

Question 10

What kind of cells forms blood islands in wall of yolk sac seeding numerous CFUs?

Answer 10

Embryonic mesoderm (mesenchymes)

Question 11

What does monophyletic theory hold?

Answer 11

All blood cells arise from a huge common stem cell called HSC (hemopoietic stem cell)

Question 12

Iron in hemoglobin is retrieved by spleen as what?

Answer 12

Ferritin

Question 13

Heme is secreted as what by gall bladder.

Answer 13

bilirubin

Question 14

Give 4 stages of monocyte development.

Answer 14

CFU-GM -> Promonocyte -> Monocyte -> Tissue-specific macrophages

Question 15

Give 4 general features of erythropoiesis.

Answer 15

Decrease in cell size, increase in heterochromatin, progressive loss of cytoplasmic organelles,
accumulation of cytoplasmic hemoglobin

Question 16

At what stage does mitosis stop?

Answer 16

Metamyelocyte (first non-mitotic stage)

Question 17

At what stage do you start to not see nucleoli?

Answer 17

Myelocyte

Question 18

Give 4 different types of macrophages!

Answer 18

Lung – alveolar macrophages, Liver – Kupffer cells, Bone – osteoclasts, Loose CT – tissue macrophages

Question 19

Colony forming unit looks like what?

Answer 19

Small lymphocyte

Question 20

What morphologically most resembles a small lymphocyte?

Answer 20

Pluripotential HSC

Question 21

CFU’s potency increases or decreases as cells become differentiated?

Answer 21

decreases

Question 22

Colony forming unit is derived from what?

Answer 22

Mesenchymal reticular tissue

Question 23

What is the difference between progenitor and precursor cells?

Answer 23

Progenitor – unipotential and self-renewing

Precursor – unipotential, but not self-renewing

Question 24

Where is the site of normal adult hemopoiesis?

Answer 24

Medullary cavity of Bone

Question 25

Which marrow is hemopoetically active?

Answer 25

Red

Question 26

Adventitial cells manufacture what?

Answer 26

Reticular fibers

Question 27

What cells are most predominant cell types undergoing development in marrow?

Answer 27

Neutrophils (RBC 2nd)

Question 28

Which marrow has reduced hemopoietic activity?

Answer 28

Yellow

Question 29

Describe the common changes that occur during differentiation into mature cell.

Answer 29

Decrease in two things, increase in six things Cell size and cytoplasmic basophilia (aka azure granule content) Nuclear heterochromatin, specific granule content, deformability, adhesiveness, phagocytic activity, and mobility

Question 30

At which stage does differentiation occur?

Answer 30

Myelocyte

Question 31

Describe what Myeloblast’s cytoplasm looks like.

Answer 31

Increasing eosinophilia, decline in azure granules, well-developed golgi

Question 32

What kind of granules does promyelocyte have?

Answer 32

Only azure granules

Question 33

At what stage do you identify specific granulocyte lineage?

Answer 33

(has specific granules) Myelocyte

Question 34

Why is granulocyte’s concentration higher in marrow than erythrocyte’s?

Answer 34

Shorter life spans

Question 35

Lymphoblasts are only cell bodies that develop both in what two tissues?

Answer 35

Lymphoid and myeloid tissues

Question 36

What is the other name for polychromatophilic erythrocyte?

Answer 36

Reticulocyte

Question 37

What characteristic feature does orthochromatophilic erythroblast have?

Answer 37

(name 2) Acidophilic cytoplasm, pyknotic nucleus, and Howell-Jolly body

Question 38

Give 3 stages of lymphopoiesis.

Answer 38

CFU-L -> lymphoblast -> lymphocytes

Question 39

What other cell does erythrocyte share their progenitor with?

Answer 39

Platelet

Question 40

What happens when you have hypoxia? (hint: induces what)

Answer 40

erythropoiesis

Question 41

How is erythropoiesis regulated?

Answer 41

Feedback loop

Question 42

Give 8 stages of erythropoiesis.

Answer 42

MEP -> CFU -> proerythroblast -> basophilic erythroblast -> polychromatophilic erythroblast -> orthochromatophilic erythroblast -> reticulocyte -> mature erythrocyte

Question 43

What is the different between promyelocyte and proerythroblast?

Answer 43

Presence of granules

Question 44

What does basophilic erythroblast remind you of?

Answer 44

Robin’s egg

Question 45

During erythropoiesis, when does cell not undergo mitosis?

Answer 45

Orthochromatophilic erythroblast stage

Question 46

What characteristic feature does polychromatophilic erythroblast have?

Answer 46

Checkerboard pattern, shifting from blue to pink

Question 47

At what stage do you not see a nucleus?

Answer 47

Poly