Hematology Slideset Two

Question 1

Answer 1

1) Rubriblast
2) Prorubricyte
3) Rubricyte
4) Metarubricyte – nucleated RBC
5) Basophilic erythrocyte with Howell Jolly body
6) Basophilic erythrocyte
7) Mature erythrocyte

Question 2

Answer 2

1) Myeloblast w/a nucleolus
2) Progranulocyte
3) Neutrophilic myelocyte
4) Neutrophilic myelocyte
5) Neutrophilic band cell
6) Monolobed neutrophil – early seg
7) Segmented neutrophil
8) Eosinophilic myelocyte
9) Eosinophilic metamyelocyte
10) Eosinophilic band cell
11) Eosinophil
12) Basophilic myelocyte
13) Basophilic metamyelocyte
14) Basophil

Question 3

Answer 3

Normal dog leukocytes

Question 4

Answer 4

Neutrophilic band

Question 5

Answer 5

Neutrophilic metamyelocyte

Question 6

Answer 6

Monocyte with vacuoles

Question 7

Answer 7

Eosinophil with granules of varying sizes, nucleus often unsegmented

Question 8

Answer 8

Large lymphocyte

Question 9

Answer 9

Small lymphocyte

Question 10

Answer 10

Neutrophil and basophil: note small nuclear thrombocytes in lower right

Question 11

Answer 11

Basophil with subtle lavender granules

Question 12

Answer 12

Basophil and Monolobed neutrophil

Question 13

Answer 13

Large clump of thrombocytes

Question 14

Answer 14

Eosinophil with rod-shaped granules

Question 15

Answer 15

Neutrophil and monocyte

Question 16

Answer 16

Lymphocyte

Question 17

Answer 17

Lymphocyte with large nucleus

Question 18

Answer 18

Eosinophil with large round granules (horse)

Question 19

Answer 19

Eosinophil and large lymphocyte (horse)

Question 20

Answer 20

Basophil and few delicate thrombocytes (horse)

Question 21

Answer 21

Monocyte (horse)

Question 22

Answer 22

Small lymphocytes

Question 23

Answer 23

Neutrophil and small lymphocyte

Question 24

Answer 24

Bone marrow comparing small lymphocytes (lower) with 2 metarubricytes immediately above lymphocyte. The uppermost cell is a lymphocyte, followed counter clockwise by a band neutrophil and free reticulum cell nucleus.

Question 25

Answer 25

A metarubricyte (lower right) is touching a small lymphocyte.

Question 26

Answer 26

Monocyte with blue-gray cytoplasm but without vacuoles.

Question 27

Answer 27

A monocyte (center cell) surrounded on either side by metamyelocyte. Cytoplasm of metamyelocyte is more red than a monocyte.

Question 28

Answer 28

Lymphocyte with slightly compressed nucleus and cytoplasm. Erythrocyte abnormalities.

Question 29

Answer 29

Crenated erythrocytes, margins appearing as sharp points. May result when blood slides dry slowly or as a normal process in aging of red cells.

Question 30

Answer 30

Basophilic stippling, blue staining granules in central erythrocyte and poikilocytes. In the dog, the presence of basophilic stippled erythrocytes and enucleated erythrocytes, in the absence of severe anemia, suggest a diagnosis of lead toxicosis.

Question 31

Answer 31

Poikilocytes, irregularly shaped erythrocytes. May be noticed in blood of anemic animals and indicate either improper production or premature destruction of erythrocytes.

Question 32

Answer 32

Rouleau (plural rouleax), erythrocytes in a roll or column. Rouleau is most pronounced in horse blood.

Question 33

Answer 33

Anisocytosis – variation in erythrocyte size; when accompanied by polychromasia erythropoiesis indicated.

Question 34

Answer 34

Acanthocytes, erythrocytes with blunt projections, thought to be a membrane defect; commonly noted in kidney disease, splenic disease (hemangiosarcoma) auto-immune hemolytic anemia and occasionally in liver disease.

Question 35

Answer 35

Spherocytes, dense spherical canine erythrocytes lacking normal biconcave shape. Polychromasia, large blue erythrocytes. Spherocytes indicate red cell membrane abnormality associated with an increased permeability to sodium ions and is associated with autoimmune mechanism hemolysis. Polychromasia indicated evidence of erythropoiesis.

Question 36

Answer 36

Reticulocytes – blue reticulum stained with new methylene blue and counterstained with Wright’s stain. Reticulocytes are the best indicator of regenerative anemia in peripheral blood.

Question 37

Answer 37

Erythrocytic inclusion bodies, a rare finding in Canine Distemper. These inclusions are generally larger than Howell-Jolly bodies and stain paler blue.

Question 38

Answer 38

Erythrocytic inclusion bodies, a rare finding in Canine Distemper. These inclusions are generally larger than Howell-Jolly bodies and stain paler blue.

Question 39

Answer 39

Rectangular erythrocyte in dog blood, rarely noted and significance unknown.

Question 40

Answer 40

Erythrocyte inclusions again, except numerous inclusions in one erythrocyte.

Question 41

Answer 41

Target cells. Irregularly shaped erythrocytes with the appearance of a target. Target cells are observed along with polychromasia in regenerative anemia. However, their appearance without polychromasia suggest the possibility of kidney, liver or splenic diseases.

Question 42

Answer 42

Heinz bodies, refractile inclusions of denatured hemoglobin in the cat. These inclusions often protrude causing bulging erythrocyte. The oxidant drug, new methylene blue, produced these changes in the cat’s erythrocytes.

Question 43

Answer 43

Heinz bodies in cat, same as previous slide except slide stained with new methylene blue.

Question 44

Answer 44

Heinz bodies and reticulocytes, stained with new methylene blue.

Question 45

Answer 45

Heinz bodies in horse caused by phenothiazine toxicity. Stained with new methylene blue. Phenothiazine is an old type dewormer still used occasionally as an additive for horse and cow dewormers.

Question 46

Answer 46

Same as previous slide but not stained with new methylene blue.

Question 47

Answer 47

Red blood cells – slight polychromasia, and anisocytosis. Note the occasional target cell.

Question 48

Answer 48

Dog bone marrow erythrogenesis.

Question 49

Answer 49

RBC’s – marked anisocytosis.

Question 50

Answer 50

RBC’s – marked hypochromasia with slight polychromasia and anisocytosis. Note the occasional target cell.

Question 51

Answer 51

Demonstrating increased number of erythrocytes with a blue reticulum, reticulocytes. Stained with new methylene blue and counterstained with Wright’s stain.

Question 52

Answer 52

RBC’s moderate anisocytosis and polychromasia with numerous Howell-Jolly bodies and metarubricytes.

Question 53

Answer 53

Many erythrocytes with abnormal projections, often thorn-like, are called acanthocytes. X1000.

Question 54

Answer 54

RBC’s – numerous target cells.

Question 55

Answer 55

Dog with Leukocytosis and Non-regenerative anemia in Myocarditis. RBC’s are morphologically normal. Leukocytes – showing a leukocytosis with a neutrophilia, a slight left shift and a monocytosis. Thrombocytes were determined to be normal by finding numerous clumps at the feather edge.

Question 56

Answer 56

Same slide as previous one. Note – no polychromasia but a rubricyte and a likely monocyte on the right.

Question 57

Answer 57

Dog with Regenerative left shift in peritonitis. RBC’s appear normal. Leukocytes slightly increased in numbers, neutrophilia with increased number of bands, cytoplasm of neutrophils is noticeable basophilic. Thrombocytes normal, clumped at feather edge. X320

Question 58

Answer 58

RBC are crenated; neutrophilic band with marked basophilia of cytoplasm. X1000

Question 59

Answer 59

Cat with degenerative left shift in pyothorax. RBC normal. Leukocytes are within normal range, yet many neutrophilic bands with basophilic cytoplasm. Note the 3 bands.

Question 60

Answer 60

Cat-leukopenia associated with subcutaneous cellulites. RBC normal. Leukocytes marked decrease in numbers, neutrophilic bands were increased and contained basophilic cytoplasm.