Flashcards in Hematopoeisis Deck (17):
Fetal hema place
hema place after birgh
yolk sac agm, fetal liver, then marrow
stem progenitor cell %
asymmetric cell division
one daughter cell becomes progenitor other remains stem cell
committed to one pathway
stem and progenitor cell identification
no distinguish morphologically, only through clonal assays
LT and ST HSC
Transplantation studies in lethally irradiated mice: LT takes longer, but can give rise to more.
common marker for stem and progenitor cells, except CFUEMk
EPO produced where>
Hematopoeisis in long bones
absent after age 25 except in proximal ends
STEM CELL PROPERTIES
multipotential, high self renewal capacity
Stem cell phenotypse (+)
CD34, tHY+, cd49 f+
STEM CELL NICHE
OSTEOBLASTIC NICHE SUPPORTS AND MAINTAINS, QUIESCNENCE OR SELF RENEWAL. VASCULAR NICHE PROVIDES SIGNALS.
Growth factor functions
produced by stromal cells. Multpiple biological activity (pleotrophic), redundancy, INTERPLAY OF SEVERAL GFS OFTEN INVOLVED, INTERACT WITH MEMBRANE RECEPTORS, COMMONLY ACT SYNERGISTICALLY, PROMOTE SURVIVAL AND DIFFERENTIATION.
early acting growth factors
SCF, FL, IL-3, GM-CSF, IL-6, and IL-11.
suppresses apoptosis of HSCs and promotes the proliferation and differentiation of stem cells, multilineage progenitor cells, and some committed progenitor cells (CFU-GEMM, GMP, CFU-Mk, BFU-E).