module 4 CBC and INtro Anemia Flashcards Preview

Hematology Module 2 > module 4 CBC and INtro Anemia > Flashcards

Flashcards in module 4 CBC and INtro Anemia Deck (38):
1

Normal RBC volume

80-100 fL

2

Acanthocyte

spur cell. irregular thorn like spikes. no central pallor. Cholesgerol preferentially trapped in outer leaflet.

3

codocytes

target cells. thin bell shaed increased surface area to volume.

4

Dacrocytes

teardrops. Round cell with a single elongated or pointed extremity; may be microcytic and/or hypochromic

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 166). Prentice Hall. Kindle Edition.

5

Drepanocyte

Round cell with a single elongated or pointed extremity; may be microcytic and/or hypochromic

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 166). Prentice Hall. Kindle Edition.

6

Echinochyte

sea urchin. Spiculated red cells with short equally spaced projections over the entire surface

7

elliptocyte

Spiculated red cells with short equally spaced projections over the entire surface

8

keratocyte

Helmet cells. Produced when a fibrin strand impales a RBC. Red cells with one or several notches with projections that look like horns on either end

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 166). Prentice Hall. Kindle Edition.

9

schistocyte

Fragments of red cells; variety of shapes including triangles, commas; microcytic

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 167). Prentice Hall. Kindle Edition.

10

spherocyte

—Spherocytic red cells with dense hemoglobin content (hyperchromatic); lack an area of central pallor

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 167). Prentice Hall. Kindle Edition.

11

stomatocyte

uniconcave red cells with the shape of a very thick cup; on stained blood smears cells have an oval or slitlike area of central pallor

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 167). Prentice Hall. Kindle Edition.

12

test for echinocyte artifact

use plastic slides as the glass releases chemicals which can cause echinocyte formation.

13

Normal RBC MCH

30 pg.

14

Only hyperchromic RBC

Spherocyte

15

Basophillic stipling

bluish black inclusions of ribosomes sometimes assoiaed with mitochondria. Artifact.

16

Cabot rings

Remnants of spindle fibers. Indicate dyserythropoesis.

17

hOWELL jOLLEY bODIES

Nuclear DNA suggest nucleaer maturation abnormalities. Individual chromosome fails to attach.

18

Heinz bodies

Denatured hemoglobin attached to band 3.

19

Sideroblasts

erythroblasts with stainabl iron granules

20

Siderocytes

non nucleated RBCS with stainable iron granules

21

Pappenheimer bodies

damages lysosomes and mitchondria

22

corrected reticulcute count

corrects for abnormally low or high hematocrit.
Patient hematocrit/ Normal hematocrit * % reticulocyte = Corrected reticulocyte count (%)

23

reticuocyte count HYPOPROLIFERATIVE ANEMIAS

62% or an absolute reticulocyte count 675 * 10 9 /L

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 184). Prentice Hall. Kindle Edition.

24

RETICULOCYTE COUNT HEMOLYTIC ANEMIA

corrected count 72% or an absolute count 7100 * 10 9 /L

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 184). Prentice Hall. Kindle Edition.

25

STRESS OR SHIFT RETICULOCYTES

RELEASED EARLIER THAN NORMAL WHEN MORE RBCS NEEDED. LARGE POLYCHROMATOPHILLIC CELLS. LONGER TO MATURE.

26

RPI

The RPI is a good indicator of the adequacy of the bone marrow response in anemia. Generally speaking, an RPI 72 indicates an appropriate bone marrow response, whereas an RPI 62 indicates an inadequate compensatory bone marrow response (hypoproliferation) or an ineffective bone marrow response.

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 185). Prentice Hall. Kindle Edition.

27

RPI CALCULATION

Patient’s hematocrit/ 0.45 L/L (normal hematocrit) * Reticulocyte count (%/ ) Reticulocyte maturation time (days)

28

IMMATURE RETICULOCYTE FRACTION

BASED ON RNA CONCENTRATION IN CELL. THROUGH FLOURESCENCE AND ABSORBANCE. IRF INCREASES BEFORE RETICULOCYTE COUNT.

29

ANISOCYTOSIS ASSOCIATED WITH:

Anemias associated with an increased RDW

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 186). Prentice Hall. Kindle Edition.

30

MICROCYTOSIS ASSOCIATED WITH:

Iron-deficiency anemia; thalassemia; sideroblastic anemia, long-standing anemia of chronic disease

31

MACROCYTOSIS ASOCIATED WITH

megaloblastic anemias; hemolytic anemia with reticulocytosis; recovery from acute hemorrhage; liver disease; asplenia; aplastic anemia; myelodysplasia; endocrinopathies; alcoholism

McKenzie, Shirlyn B.; Williams, Lynne. Clinical Laboratory Hematology (3rd Edition) (Pearson Clinical Laboratory Science Series) (Page 186). Prentice Hall. Kindle Edition.

32

INCREASED UNCONJUGATED BILLIRUBIN

HEMOLYSIS EITHER iv OR EV.

33

INCREASED IV HEMOLYSIS

11-9). Hemosiderin in urine (hemosiderinuria), decreased plasma haptoglobin and hemopexin (as a result of increased consumption), and increased methemalbumin

34

ERYTHROCYTE SURVIVAL STUDY

SMALL AMOUNT OF BLOOD REMOVED, RADIOLABELED. CHROMIUM IS TRAPPED. REINJECTED, AND DRAWN PERIODICALLY.

35

MASS =

PRODUCTION * SURVIVAL

36

COMPENSATION FOR HYPOXIA / ANEMIA

INCREASE IN 2-3 BPG DECREASED OXYGEN AFINITY DUE TO BOHR EFFECT

37

MCH

HB / RBC COUNT

38

MCHC

HB / HEMATOCRIT