Hemocytometry Flashcards
(18 cards)
What is the markings at the top of the bulb in White cell pipette?
11
What is the markings at the top of the bulb in Red Cell Pipette?
101
What is the total area lf Neubauer ruling?
9mm^2, divided into 9 large sq. (1mmx1mm)
corner squares: WBC divided into?
16 smaller squares (0.25mm)
Central square RBC divided into?
25 smaller squares (0.04mm^2)
Distance between coverslip and counting chamber?
0.1mm
Total volume of one counting area
0.9mm^3
In fuch’s- Rosenthal Hemocytometer, how many counting chambers?
2
In Fuch’s-Rosenthal Hemocytometer, each counting chamber has a total square of?
16mm^2 divided into 16 large squares (1mmx1mm)
depth=0.2mm
Each large square is further divides into how many smaller squares?
16 smaller squares (0.25mmx0.25mm)
How many counting chamber has Spiers-Levy hemocytometer?
4 counting chambers
In Spiers-Levy, each counting chamber has a total area of?
10mm^2 divided into 10 large squares
What is the arrangement of large squares in Spiers-Levy? what is the measurement?
Horizontal,1mmx1mm
In Spiers-Levy, Each large square is divided into?
16 smaller squares (0.25mmx0.25mm)
In diluting fluids, what is the characteristic of RBC?
isotonic, prevent coagulation of red cells
In diluting fluids, what is the characteristic of WBC?
weak acid solutions used to lyse red cells to facilitate white cell counts
Same procedure as RBC count but using WBC thoma pipette, draw blood to the ____ mark?
0.5 mark
Dilution ratio in RBC count but using WBC thoma pipette
1:20
