HEMOGLOBINOMETRY

Question 1

most common anticoagulant for routine hematology testing

Answer 1

ethyldiaminetetraacitic acid (edta)

Question 2

what tests has minimal effects on blood cell morphology

Answer 2

complete blood count
differential count

Question 3

how long does CBC testing should be stored if it is stored at RT and if it is stored at 4’C

Answer 3

6hrs - RT
24hrs - 4’C

Question 4

why peripheral blood films should be prepared within 3hrs?

Answer 4

reduce cell detoriation and morphology artifacts

Question 5

may be necessary to use manual methods when counts exceed the linearity of an instrument

Answer 5

manual cell count

Question 5

equipment used in manual cell count

Answer 5

hemacytometer

Question 6

purpose of platelet count

Answer 6

to count the platelets in a 1 liter of blood

Question 6

lyses the nonnucleated rbcs in the specimen to prevent interference in the count

Answer 6

diluting fluid

Question 6

purpose of wbc count

Answer 6

to count the wbc’s in a 1 liter of blood

Question 6

diluting fluid used in wbc count

Answer 6

1% buffered ammonium oxalate

Question 7

ratio of platelet to diluting fluid

Answer 7

1:100

Question 7

they are small and can be confused easily with dirt or debris

Answer 7

platelet

Question 7

ratio of wbc to diluting fluid

Answer 7

1:20

Question 7

diluting fluid used in platelet count

Answer 7

1% ammonium oxalate

Question 8

the adherence of platelets found around neutrophils, producing a ring or satellite effect. what anticoagulant is used in this phenomenon.

Answer 8

platelet satellitosis; edta

Question 9

what anticoagulant should correct the platelet satellitosis

Answer 9

sodium citrate

Question 10

more accurate manual rbc procedures

Answer 10

hematocrit
hemoglobin concentration

Question 10

rarely performed because of the innacuracy of the count and questionable necessity

Answer 10

red blood cell count

Question 11

commercially available for wbc and platelet counts

Answer 11

capillary pipette
diluent reservoir systems

Question 11

carry oxygen to and carbon dioxide to the tissues

Answer 11

hemoglobin

Question 12

it consists of a capillary pipette that fits into a plastic reservoir containing 1.98% ml of 1% buffered ammonium oxalate

Answer 12

Leuko Chek

Question 13

what is the absorbance of cyanmethemoglobin and is it direct or indirectly proportional to the hemoglobin concentration

Answer 13

540nm; directly proportional

Question 14

this reagent is sensitive to light and it should be stored in a brown bottle

Answer 14

cyanmethemoglobin reagent

Question 15

what ingredient of the hemoglobin reagent is highly toxic and must be used cautiously

Answer 15

cyanide

Question 16

volume of packed rbcs that occupies a given volume of whole blood

Answer 16

hematocrit

Question 17

this instrument uses precalibrated capillary tubes and has built-in hematocrit scales, which eliminates the need for separate reading devices

Answer 17

READACRIT centrifuge

Question 18

This rule applies only to specimens that have normcytic normochromic rbcs

Answer 18

rule of three

Question 18

what are the redd blood cell indices

Answer 18

mean cell volume mean cell hemoglobin mean cell hemoglobin concentration

Question 19

eliminates the use of sealants

Answer 19

SUREPREP capillary tubes

Question 20

average volume of rbcs expressed in femtoliters

Answer 20

mean cell volume

Question 20

average weight of hemoglobin in a RBC, expressed in picograms

Answer 20

mean cell hemoglobin

Question 21

average concentration in each individual rbc. the units used are grams per deciliter

Answer 21

mean cell hemoglobin concentration

Question 22

last immature rbc stage

Answer 22

reticulocyte

Question 22

precipitated hemoglobin, usually appear round or oval, and tend to adhere to the cell membrane

Answer 22

heinz bodies

Question 23

round nuclear fragments and usually singular

Answer 23

howell-jolly bodies

Question 23

iron in the mitochondria whose presence can be confirmed with an iron stain, such as prussian blue

Answer 23

pappenheimer bodies

Question 23

reticulocytes that are released from the marrow prematurely

Answer 23

shift reticulocytes

Question 24

useful in detecting early hematopoietic activity after chemotheraphy or hematopoietic stem cell transplantation

Answer 24

Immature Reticulocyte Fraction (IRF)

Question 25

useful to detect early iron deficiency

Answer 25

reticulocyte hemoglobin

Question 25

tests to detect and monitor the course of inflammatory conditions such as rheumatid arthritis, infections,or certain malignancies

Answer 25

erythrocyte sedimentation rate

Question 26

not a specific test for inflammatory diseases and is elevated in many other conditions also it is not recommended as a screening test to detect inflammatory conditions in asymptomatic individuals

Answer 26

erythrocyte sedimentation rate

Question 27

most commonly used method today that the taller column height allows the detection of highly elevated esrs

Answer 27

modified westergren erythrocyte sedimentation rate

Question 28

offers the ability to produce rapid and accurate results that facilitate faster treatment, which can decrease hospital length of stay

Answer 28

point -of-care testing

Question 28

this method uses oxalate-anticoagulated whole blood

Answer 28

wintrobe erythrocyte sedimentation rate

Question 29

means that regardless of where the diagnostic testing is performed or who performs the test, all testing sites must follow the same regulatory requirements based on the complexity of the test

Answer 29

testing site neutrality

Question 30

useful in selecting equipment that best covers the patient population for the institution

Answer 30

patient correlation studies

Question 31

use the conductivity method to determine the hematocrit

Answer 31

i-STAT 1 and Epoc

Question 32

instrument that eliminates the interference from turbidity in the specimen and what is its waveleghths

Answer 32

HemoCue hemoglobinometer; 570nm and 880nm

Question 33

used to detect indirectly evaluate the oxygen carrying capacity of the blood

Answer 33

hemoglobin estimation

Question 34

physiological factors in hemoglobin estimation

Answer 34

age gender pregnancy physical activity or exercise

Question 35

pathological factors in hemoglobin estimation

Answer 35

testosterone deficiency renal deficiency myelodysplasia diminished erythrocyte bone marrow suppresion blood loss

Question 36

most accurate method for estimation of hb

Answer 36

cyanmethemoglobin method

Question 37

specimens used in cyanmethemoglobin method

Answer 37

edta anticoagulated blood capillary blood

Question 38

this method is based on the capacity of hemoglobin to combine with the same maximum volume of carbon dioxide as of oxygen

Answer 38

gasometric method

Question 39

this method or technique of measuring hemoglobin is not routinely performed except as a method of screening blood donors

Answer 39

gravimetric method

Question 40

hydrochloric acid and hemoglobin produces what and what is its color

Answer 40

acid hematin; brown hematin