hw questions

Question 1

which DNA mutagen results in a transversion mutation

Answer 1

oxygen radicals

Question 2

which DNA mutagen results in a frameshift mutation

Answer 2

acridine orange

Question 3

in its common form, the keto form, 5-bromodeoxyuridine is an analog of what nucleotide?

Answer 3

thymine

Question 4

in drosophila, which cross results in hybrid dysgenesis

Answer 4

P cypotype males to M cyptopye females

Question 5

what is the transposable elements that acts as a control element in maize?

Answer 5

Ds

Question 6

Ac elements in yeast contain which gene?

Answer 6

transposase

Question 7

the transposable element that is the cause of the X-linked blood-clotting disorder hemophilia A is a

Answer 7

LINE element

Question 8

all bacterial transposable elements have a gene that encodes which of the following enzymes?

Answer 8

transposase

Question 9

which hereditary condition is a consequence of insertional inactivation?

Answer 9

hemophilia A

Question 10

what are tautomers of nucleotide bases

Answer 10

isomers that differ from each other in the location of one hydrogen atom in the molecule

Question 11

what nucleotide will base-pair with the enol form of 5-bromouracil?

Answer 11

guanine

Question 12

purpose of the Ames test

Answer 12

test the mutagenic effects of chemicals

Question 13

in the Ames test, the appearance of his+ revertants in the presence of a non-mutagenic control compound indicates that

Answer 13

some of the reversion mutations aren’t caused by the mutagen being tested

Question 14

true or false: many chemicals are more mutagenic after being processed in the liver

Answer 14

true

Question 15

true or false: all compounds that have been found to be mutagenic in the Ames test are also carcinogenic

Answer 15

false

Question 16

why are liver extracts used in the Ames test?

Answer 16

liver enzymes may activate some innocuous compounds, making them mutagenic

Question 17

which bacteria grow on the agar plate if the Ames test is positive?

Answer 17

his+ prototrophs

Question 18

to use RFLP analysis to detect a SNP, the SNP must

Answer 18

occur within a restriction enzyme recognition sequence

Question 19

molecular tests for Huntingtons, cystic fibrosis, and tay-sachs are possible because…

Answer 19

sufficient info gathered on DNA sequences of both the mutant and normal alleles

Question 20

what is 5BU best described as

Answer 20

a base analog that can cause either A-T > G-C or G-C > A-T transitions

Question 21

for 5BU to cause a transition mutation, which of the following must occur

Answer 21

DNA with incorporated 5BU must replicate

Question 22

true or false: in its rare form, 5BU pairs with guanine

Answer 22

true

Question 23

what are the consequences of having pyrimidine dimers in DNA

Answer 23

they distort the DNA structure and result in errors during DNA replication

Question 24

branch migration

Answer 24

process that determines the length of heteroduplex DNA on chromatids

Question 25

what process does not occur during recombination?

Answer 25

DNA polymerization

Question 26

what is not a characteristic of IS elements

Answer 26

copy of IS becomes integrated at a new location

Question 27

what trinucleotide repeat disorder isn't consistent with hairpin formation?

Answer 27

Friedreich ataxia (GAA)

Question 28

if non-trinucleotdie repeat expansions were present in the coding region of a gene, which mutation is the most likely?

Answer 28

frameshift

Question 29

which type of mutagen has a structure similar to one of the DNA nucleotides and therefore can work its way into DNA, where is pairs with a nucleotide?

Answer 29

nucleotide base analog

Question 30

which of the following is a consequence of inactivation of both p53 alleles in a cell?

Answer 30

reduction of p21 synthesis

Question 31

can p53 bind to DNA lesions?

Answer 31

no

Question 32

true or false: Alu elements are SINEs that typically encode an important human protein: restriction endonuclease Alu I

Answer 32

false

Question 33

is P- cis or trans acting?

Answer 33

cis

Question 34

what technique can be used to identify binding location of Lac repressor protein relative to location of RNA polymerase binding in the regulatory region of the lac operon?

Answer 34

DNase I footprint protection analysis

Question 35

how does the phage know if bacteria are actively growing?

Answer 35

protein cII is degraded by proteases

Question 36

binding cII/cIII to PL triggers the expression of what leading to the lysogenic cycle?

Answer 36

integrase

Question 37

if the CAP binding region is mutated, what effect would you expect to observe?

Answer 37

the lac operon will exhibit a basal level of transcription

Question 38

which yields the least amount of transcription of the trp operon

Answer 38

trpP-/tryptophan absent

Question 39

which yields the most amount of transcription of the trp operon

Answer 39

trp+/tryptophan absent