Hybridoma Technology

Question 1

Hybridoma Technology is discovered by

Answer 1

Kohler and Melstein

Question 2

Kohler and Milstein uses ____ to fused Myeloma cells and Spleen cells

Answer 2

Sendai Virus

Question 3

In order to produced a Hybridoma Cells fusion of ___ and ___ is needed

Answer 3

Myeloma Cells
Spleen Cells (from mouse)

Question 4

Immortal cells that produces Antibodies

Answer 4

Hybridoma cells

Question 5

Purified antibodies; with exceptional purity and specificity that can only react with specific antigen (single epitope)

Answer 5

Monoclonal antibodies

Question 6

First step in making Hybridoma Cells

Answer 6

Allowing mouse to be immunized with an antigen to produce Spleen Cells

Question 7

In the presence of _____ myeloma cells and spleen cells are fused

Answer 7

Polyethylene glycol

Question 8

After fusion cells are placed in the medium containing __ to allow the growth of Hybridoma cells

Answer 8

HAT

Hypoxanthine
Aminopterin
Thymidine

Question 9

Clinical Applications of Hybridoma Technology

Diagnosis

Answer 9

Pregnancy
Measurement of Hormone Levels
Tumor Ag detection

Question 10

Clinical Applications of Hybridoma Technology

Therapeutics

Answer 10

Cancer
Autoimmune disease
- Influximab (inhibits TNF-a)