IBX - Fermentation Reactors Flashcards by Vince Joell Feratero

Yield coefficient represents

total biomass or product produced
conversion efficiency of a substrate into product
conversion rate of a substrate into biomass or product
production time of biomass or produc

conversion efficiency of a substrate into product

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The lowest biomass yield in a culture of Escherichia coli will be in

an aerated batch culture containing a initial high concentration of glucose
an aerated batch reactor containing an initial low concentration of glucose
an aerated fed-batch reactor having a low glucose concentration
an aerated continuous reactor having a low glucose concentration

an aerated batch culture containing a initial high concentration of glucose

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When two populations compete for a single growth limiting substrate in a continuous fermenter, which organism would not be washed out?

Organism maintaining the highest substrate concentration
Organism maintaining the lowest substrate concentration
Both (a) and (b)
Organism maintaining the moderate substrate concentratio

Organism maintaining the lowest substrate concentration

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The continuous cultures are not widely used in industry because

they are not suited for the production of secondary metabolites
contamination or mutation can have a disastrous effect on the operation
the government will not approve the licensing of pharmaceuticals produced in continuous cultures
all of the above

all of the above

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The lowest yield of ATP /is in

fermentation
aerobic respiration
anaerobic respiration
same in (a), (b) and (c)

fermentation

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Mixing in an anaerobic sludge blanket reactor is due to

rapid change in water temperatures throughout the reactor
release of gases by the microbial populations
swimming of microbes
none of the above

release of gases by the microbial populations

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In batch culture, protogon is produced from peptone during the stationary phase with a yield of 0.4 protogon mg per g of peptone. If it is to be produced in a chemostat at a dilution rate of 0.5 h-1 from a medium containing 10 g.l-1 of peptone, then the rate of protogon synthesis would be

0 g.l-1h-1
0.5 g.l-1h-1
1 g.l-1h-1
2 g.l-1h-1

0 g.l-1h-1

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Formation of end product by Lactococcus lactis will become non-growth associated as lactic acid accumulates because

cells will redirect ATP to anabolism
cells will redirect NAD+ to anabolism
cells will redirect ATP to facilitate the diffusion of lactic acid and H+ out of the cells
cells will redirect ATP to the active transport of lactic acid and H+ out of the cells

cells will redirect ATP to the active transport of lactic acid and H+ out of the cells

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Which of the following would not be subjected to the “glucose effect”?

Aspergillus fumigatus
Saccharomyces cerevisiae
Escherichia coli
Aspergillus niger

Escherichia coli

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Immobilized cell reactors for wastewater treatment have the advantage of having/being

higher cell concentration
more stable and prevent washout
higher dilution rate before the cells washout
all of the above

all of the above

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Which of the statement is true for continuous reactor at steady state?

The rates of biomass, substrate and product concentrations are zero
Biomass, substrate and product concentrations are zero
Biomass, substrate and product concentrations do not change with time
Biomass, substrate and product concentrations change with time

Biomass, substrate and product concentrations do not change with time

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A continuous bioreactor in which only the flow rate is used to control the rate of cell or product productivity is called

turbidostat
chemostat
level stat
pH

chemostat

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For organisms growing in a chemostat, the specific growth rate

cannot be determined
can be determined from the dilution rate
equals to the maximum specific growth rate of the culture
none of the above.

can be determined from the dilution rate

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Saccharomyces cerevisiae is being grown in a chemostat converts glucose to biomass, ethanol, glycerol and carbon dioxide. At steady state, the concentration of glucose, biomass, ethanol and glycerol will

decrease with time
increase with time
be constant
change randomly with time

be constant

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In aerobic yeast fermentation for production of citric acid from alkanes using a fed-batch culture, why alkanes are slowly fed to the yeast?

Citric acid is toxic to the cells
Alkanes cause foaming
Fast addition of alkanes will inhibit the cells and reduce oxygen transfer rates
Fast addition of alkanes will cause the cells to grow too quickly

Fast addition of alkanes will inhibit the cells and reduce oxygen transfer rates

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A continuous reactor has a dilution rate of 0.5 h-1. Its residence time would be

ln(2)/0.5
ln(2) x 0.5
0.5 h
2 h

2 h

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Which of the following is used to calculate mass of substrate in the reactor?

Flow rate x substrate concentration in the reactor
Volume of reactor x substrate concentration in reactor
Flow rate x mass of reactor
Volume of reactor x Flow rate

volume of reactor x substrate concentration in reactor

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If biomass yields are constant, then the biomass productivity of a culture grown in continuous reactor will

always decrease with dilution rate
increase with dilution rate until washout
remain constant irrespective of the dilution rate
decrease with dilution rate until washout

increase with dilution rate until washout

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Low dissolved oxygen concentrations leads to

low biomass yields
high biomass yields
no effect on biomass yields
none of the above

low biomass yields

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In a continuous reactor, the medium contains 40 g.l-1 of maltose and the medium flow rate is 10 litres per hour and the effluent contains 20 g.l-l of lactate. What is the productivity of lactate production from this reactor?

50 g maltose.l-1
50 g lactate.l-1
200 g maltose.l-1
200 g lactate.l-1

200 g lactate.l-1

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A fed-batch reactor containing 1 g.l-1 of substrate in 2 litre of medium. It is fed with 1 g.l-l of substrate at the rate of 1 litre per hour. After 10 hours, the concentration of substrate in the reactor was 0.5 g.l-1 . The mass of substrate that was used by the culture in the reactor was

2g
6g
8g
12g

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Toxins accumulated in a reactor can lead to low biomass yields probably due to which of the following?

NAD+ is diverted away from homeostasis to anabolism
ATP is diverted away from homeostasis to anabolism
NAD+ is diverted away from anabolism to homeostasis
ATP is diverted away from anabolism to homeostasis

ATP is diverted away from anabolism to homeostasis

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The cellular productivity in a continuous stirred tank fermenter (CSTF) increases with an increase in the dilution rate and reaches a maximum value. If the dilution rate is increased beyond the maximum point, the productivity will

decrease abruptly
increase
increase drastically
be zero

decrease abruptly

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Fluidized bed bioreactors provide higher mass transfer rates than packed bed bioreactors because

mixing is higher in fluidized bed bioreactors
particles move with the fluid in a fluidized bed bioreactor
immobilized particles are smaller in the fluidized bed bioreactors
all of the above

particles move with the fluid in a fluidized bed bioreactor

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In fed-batch fermentation, mass of substrate utilized is calculated using the relationship initial mass of substrate in the reactor + final mass of substrate in the reactor + flow rate x [substrate in the feed] initial mass of substrate in the reactor - final mass of substrate in the reactor + flow rate x [substrate in the feed] initial mass of substrate in the reactor + final mass of substrate in the reactor - flow rate x [substrate in the feed] initial mass of substrate in the reactor + final mass of substrate in the reactor - flow rate

initial mass of substrate in the reactor - final mass of substrate in the reactor + flow rate x [substrate in the feed]

A chemostat has a liquid volume of 2 litres and is being fed at a rate of 4 litres per hour. Dilution rate for this reactor will be 2 litres 2 litres per hour 2 h-1 4 litres per hour

2 h-1

Mixing per unit volume is observed to be poorest in continuous packed bed reactor continuous fluidized bed bioreactor continuous airlift bioreactor none of the above

continuous packed bed reactor

The growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h-1 and Ks =0.1 g.l-1, if the concentration of glucose in the feed is 10 g.l-1 and the dilution rate is set to 0.4 h-1, then the steady state concentration of glucose in the effluent will be 0 g.l-1 0.5 g.l-1 1.0 g.l-1 10 g.l-1

0.5 g.l-1

A continuous reactor at steady state contains 0.04 g.l-1 of biomass and 0.02 g.l-1 of phenol. The feed contained 0.1 g.l-1 of phenol. The biomass yield would be 0.4 g.g-1 0.5 g.g-1 1.0 g.g-1 5.0 g.g-1

0.5 g.g-1

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system. continuous batch fed-batch semi continuous

continuous

The main reason for production of antibiotics in fed batch reactors is the presence of precursors is often toxic to the cells higher yields when cells enter the stationary phase higher yields when cell growth slows all of the above

all of the above

Fed batch reactors is used to produce vinegar because it can maintain low ethanol concentrations it can maintain low acetic acid concentrations acetic acid bacteria tend to ferment at high ethanol concentrations all of the above

it can maintain low ethanol concentrations

When a culture of fermenting yeast becomes metabolically uncoupled, ethanol becomes a secondary metabolite production becomes growth associated production becomes non-growth associated becomes a growth nutrient

production becomes non-growth associated

Mixing profiles closest to plug flow are observed in continuous packed bed reactor stirred tank reactor with biomass recycles continuous fluidized bed bioreactor all of these

continuous packed bed reactor

On a glucose medium, the growth of an organism is characterized by the following Monod model and stoichiometric parameters μm = 0.3 h-1, Ks =0.05 g.l-1 and Yxs = 0.3 g.g-1 When this organism is grown in a 2 litre continuous on a medium containing 10 g.l-1 of glucose added at 1 l.h-1, the steady state concentration of biomass in the reactor will be 0 g.l-1 0.4 g.l-1 0.5 g.l-1 10 g.l-1

0 g.l-1

Unsteady state mass balance for dynamic model of continuous stirred tank reactor (CSTR) is time rate of total amount in rector = rate of addition to reactor -rate of removal + rate of formation total amount in rector = rate of addition to reactor - rate of removal + rate of formation rate of total amount in rector = rate of addition to reactor - rate of removal + rate of formation none of the above

time rate of total amount in rector = rate of addition to reactor -rate of removal + rate of formation

A fed-batch reactor initially contains 2 litre of medium. If it was fed at 1 litre per hour, then after 10 hours, the volume of the reactor will be 1 litre 2 litre 3 litre 13 litre

2 litre

A Bacillus sp. produces an antibiotic only during the stationary phase when grown in a batch culture. If grown in a 5 litre continuous culture, the steady state productivity of the antibiotic will increase until the culture washes out will decrease after washout will be zero will be infinite

will be zero

In a fluidized bed reactor, the function of the disengagement zone is to reduce loss of particles from the reactor reduce the velocity of the particles near the surface of the reactor reduce foaming problems all of the above

all of the above

Saccharomyces cerevisiae produces high biomass yields at low glucose concentrations and high dissolved oxygen concentrations. Which of the following should be followed for maximizing its biomass productivity? Batch fermenter with a high initial glucose concentration Continuous fermenter with a low initial glucose concentration Fed batch fermenter All of the above

Fed batch fermenter

In a glucose-yeast extract medium, Lactococcus lactis has a maximum specific growth rate (um) of 1.23 h-1. What would be the specific growth rate of this organism at steady state in a 4 litre reactor being fed at 2 litres per hour? 0.5 h-1 1.2 h-1 2.4 h-1 4 h-1

0.5 h-1

Which of the following is not correct with regards to continuous cultures? The major industrial scale application of continuous cultures is in waste water treatment Mutation and contamination present a major risk to the operation of continuous cultures for the production of metabolites The continuous cultures is very useful for the production of pharmaceuticals Continuous cultures have an advantage that they do not need to shut down as regularly as batch culture reactor

The continuous cultures is very useful for the production of pharmaceuticals

In a plug flow reactor, the highest concentration of substrates is exposed to the cells that are near the effluent or exit of the reactor are in the mid way along the reactor are located near entrance of feed are near the wall of the reactor

are located near entrance of feed

In an activated sludge process, the biomass is recycled to increase the efficiency of the process reduce sludge volumes increase the concentration of cells all of the above

all of the above

A strain of Escherichia coli has a maximum specific growth rate of 0.8 h-1 on a glucose based medium. If this organism is being grown in a chemostat with a dilution rate of 1.2 h-1, then at steady state the concentration of E. coli in the same medium will increase be zero change randomly decrease

be zero

A fed-batch reactor containing 2 litre of medium and 0.1 g.l-l of biomass is fed with a medium containing 1 g.l-1 of substrate at 1 litre per hour. If after 10 hours, the concentration of biomass in the reactor was 0.2 g.l-1, then the biomass produced during the 10 hour period is 1.5 g 2.2 g 3.0 g 6.0 g

2.2 g

Which of the following organisms will continue their metabolism at high substrate concentrations? Pseudomonas aeruginosa Saccharomyces cerevisiae Bacillus subtilis Escherichia coli

Pseudomonas aeruginosa

Yeast is typically produced in fed batch reactors because yeast cells produce ethanol at low glucose concentrations yeast cells ferment at low glucose concentrations yeast cells respire at low glucose concentrations all of the above

yeast cells respire at low glucose concentrations

Two continuous bioreactors containing the same organisms, fed with the same feed at the same dilution rate were compared. Reactor 1 started with an initial concentration of glucose of 10 g.l-1, while reactor 2 contained 0.1 g.l-1 of glucose at the start of the process then at steady state the concentration of glucose in reactor 1 would be greater than that in reactor 2 the concentration of glucose in reactor 1 would be equal to reactor 2 the concentration of glucose in reactor 1 would always be zero. the concentration of glucose in reactor 1 would be less than that in reactor 2

the concentration of glucose in reactor 1 would be equal to reactor 2

In a fluidized bed reactor mixing is achieved by gravity flow of fluid upward movement of the incoming feed diffusion both (a) and (b)

both (a) and (b)

The production of an amylase from starch by Aspergillus niger is often performed in a fed batch fermenter. This is due to the reason that fed-batch reactor can be used to minimize starch concentrations and prevent a. niger from fermenting fed-batch reactor can be used to minimize starch concentrations and thus reduce the viscosity of the medium continuous reactor can be used to minimize starch concentrations and their inhibitory effects all of the above

fed-batch reactor can be used to minimize starch concentrations and thus reduce the viscosity of the medium

The fastest route of NADH reoxidation is aerobic respiration anaerobic respiration fermentation decomposition

fermentation

In fed batch bioreactor modelling, the rate of change in the bioreactor volume is assumed to be equal to flow rate initial volume volume of solids in the reactor none of these

flow rate

Monoclonal antibodies are typically produced in fed batch reactors because hybridoma cells ferment at high maltose concentrations hybridoma cells yield higher lactate at high glucose concentrations hybridoma cells respire at high glucose concentrations none of the above

hybridoma cells yield higher lactate at high glucose concentrations

In plug flow tubular reactor the axial velocity profile on cross-section will be constant varying with time non-linear power function of time

constant

Production of organic acids in batch culture is generally growth associated phase followed by non-growth associated production. The probable reason for this is that organic acids uncouple catabolism from anabolism biomass yields increase as the fermentation proceeds organic acids are secondary metabolites high concentrations of organic acid promote the growth of the microbial population

organic acids uncouple catabolism from anabolism

The concentration of biomass in a continuous stirred tank reactor is 20 g dry weight per litre. What would be the concentration of biomass in the effluent? Greater than 20 g dry weight per litre Approximately 20 g dry weight per litre Lesser than 20 g dry weight per litre More than 30 g dry weight per litre

Approximately 20 g dry weight per litre

The ideal tubular-flow fermenter without radial variations is called a plug flow fermenter continuous stirred tank fermenter (CSTF) column fermenter any intensity of dilution rate near the wash out points

plug flow fermenter

Pseudo-steady state in a continuous reactor refers to a reactor in which Pseudomonads are being cultured concentration oscillate around a mean value concentration increase with time concentration decrease with time

concentration oscillate around a mean value

The toxicity of organic acids to cells enhances as the pH falls reduces as the pH falls is not affected by pH is highest at pH 7.0

enhances as the pH falls

Which of the following is best described as secondary metabolite? Acetic acid produced from the oxidation of ethanol Ethanol from the fermentation of glucose Penicillin Citric acid from the partial oxidation of glucose

Penicillin

A microorganism which is subjected to the Crabtree effect is most likely to ferment in the presence of low concentrations of sugar and dissolved oxygen respire in the presence of low sugar and high dissolved oxygen concentrations ferment in the presence of high concentrations of sugar and dissolved oxygen respire in the presence of high sugar and low dissolved oxygen concentrations

ferment in the presence of high concentrations of sugar and dissolved oxygen

When the product formation is approximately equal to the rate of cell growth, the pattern of product formation is termed as uncoupled growth associated non-growth associated metabolically uncoupled

growth associated

Which of the following would be correct concerning the concentration of biomass (X). growth limiting substrate (S) and product (P) in a chemostat when the cells have been washed out? X,S and P will be zero X,S and P can not be predicted for the above condition X and P will be zero while S is equal to the substrate concentration in feed None of the above

X and P will be zero while S is equal to the substrate concentration in feed

Pseudomonas aeruginosa has a maximum specific growth rate of 0.8 h-1 in a medium with glucose as a growth limiting substrate. To prevent wash out, the dilution rate must be set to greater than 0.8 h-1 less than 0.8 h-1 equal to 0.8 h-1 1.6 h-1

less than 0.8 h-1

Saccharomyces cerevisiae when grown in high glucose concentration medium, the cells activity depends on which of the following? High concentration of dissolved oxygen Low concentration of dissolved oxygen No effect of dissolved oxygen High concentration of dissolved oxygen and sodium salts

No effect of dissolved oxygen

Continuous culture has an advantage over to a batch cultures as continuous reactors do not need to be shut down and cleaned as regularly as a batch reactor most downstream and upstream processes are continuous in nature and thus the operation of continuous bioreactor can be better integrated into the overall manufacturing process cells can be maintained at a constant physiological state simply by setting the dilution rate all of the above

all of the above

In the batch-fed mode, substrate will be added only at the beginning of the fermentation process periodically throughout the fermentation process only at the end of the fermentation process substrate is never added

only at the end of the fermentation process

When Escherichia coli grow in a well aerated medium, which of the following will occur? Fructose di-phosphate accumulation inside the cells leading to cell respiration Fructose di-phosphate accumulation inside the cells leading to fermentation Fructose di-phosphate will accumulate regardless of the medium composition Fructose di-phosphate will accumulate inside the cells leading to anaerobic respiration

Fructose di-phosphate accumulation inside the cells leading to fermentation