ID OF BACTERIACEAE

Question 1

Ability to ferment glucose, lactose, and sucrose and to
produce hydrogen sulfide

Answer 1

Triple Sugar Iron Agar (TSI)

Question 2

Composition of TSI agar

Answer 2

: 1% lactose, 1% sucrose, 0.1% glucose

Question 3

what is added in TSI to detect the production of hydrogen sulfide

Answer 3

Ferrous sulfate and sodium thiosulfate

Question 4

pH indicator in TSI agar

Answer 4

phenol red

Question 5

orginal color of the TSI agar

Answer 5

red

Question 6

is a similar medium to TSI, but only
incorporates the carbohydrates glucose and lactose.
- H2S production can also be detected with this
formulation

Answer 6

Kligler’s iron agar (KIA)

Question 7

• Phenol red is used as the pH indicator which is
  yellow below the pH of ___. An inoculated medium is red because the pH is buffered at _____

Answer 7

6.8, 7.4.

Question 8

glucose fermentation in TSI

Answer 8

ALK SLANT/ACID BUTT or K/A

Question 9

IMViC stands for

Answer 9

● Indole Test
● Methyl Red Test
● Voges-Proskauer Test
● Citrate Utilization Test

Question 10

Test the ability of the bacteria to produce the
enzyme tryptophanase and deaminate
tryptophan to indole, pyruvic acid, and
ammonia

Answer 10

INDOLE TEST

Question 11

Test that deaminate TRYPTOPHAN

Answer 11

INDOLE TEST

Question 12

ENZYME OF INDOLE TEST

Answer 12

tryptohanase

Question 13

Production of indole is detected using

Answer 13

Ehrlich’s reagent or Kovac’s reagent

Question 14

Positive reaction of the two mentioned reagent on the previous card

Answer 14

pink/red, red ring at the top

Question 15

If glucose is metabolized by the mixed acid
fermentation pathway, stable acid end
products are produced, which results in a low
pH

Answer 15

METHYL RED TEST

Question 16

Positive reaction of methylk red test

Answer 16

red color

Question 17

In TSI, When sugars are fermented, acid production will occur turning the original color of the medium red into
_____

Answer 17

yellow

Question 18

Format of writing TSI results

Answer 18

Slant/butt

Question 19

How many hours is the TSI should be read before erroneous result override

Answer 19

18-24 hrs

Question 20

What does red/red mean in TSI

Answer 20

No fermentation = Alkaline/alkaline

Question 21

What does red/yellow mean in TSI

Answer 21

Glucose fermentation alkaline/acid

Question 22

what does yellow/yellow mean in TSI

Answer 22

lactose fermentation

Question 23

what dies blackening if the medium mean in TSI

Answer 23

Hydrogen sulfide production

Question 24

in TSI if the medium has bubbles then the organism is called ___

Answer 24

aerogenic

Question 25

among the 2 reagents in Indole test, which one is more sensitive?

Answer 25

Ehrlich's reagent

Question 26

what is added in Methyl red test?

Answer 26

MR pH indicator

Question 27

_____ and _______ detects the end products of glucose fermentation

Answer 27

Methyl Red and Voges-Proskauer Test

Question 28

It measure the production of acetoin

Answer 28

VOGES-PROSKAUER TEST

Question 29

VOGES-PROSKAUER TEST are measrued after addition of ____

Answer 29

alpha-naphthol followed by 40% KOH or NaOH (potassium or soddium hydroxide)

Question 30

in VOGES-PROSKAUER TEST, acetoin is oxidized into

Answer 30

diacetyl 2,3-Butanediol

Question 31

positive reaction in VOGES-PROSKAUER TEST

Answer 31

red complex

Question 32

It determines whether an organism can use sodium citrate as a sole carbon source

Answer 32

CITRATE UTILIZATION TEST

Question 33

carbohydrates source in citrate utilization test

Answer 33

sodium citrate

Question 34

medium used in citrate utilization test

Answer 34

Simmons’ Citrate Medium (SCA)

Question 35

nitrogen source in citrate utilization test

Answer 35

ammonium salts

Question 36

the utilization of this nitrogen salt results in the release of ___ causing a pH change

Answer 36

ammonia

Question 37

pH indicator in citrate iuutiliozation test

Answer 37

bromothymol blue

Question 38

positive reaction in citrate utilization test

Answer 38

blue

Question 39

Determines whether an organism has the ability to reduce nitrate to nitrite

Answer 39

nitrate reduction test

Question 40

positive reaction in nitrate reduaction test

Answer 40

red , diazo red dye

Question 41

Nitrate reduction test is a test that determines the production of an enzyme called ____

Answer 41

nitrate reductase,

Question 42

what is added in nitrate reduction test after incubation

Answer 42

N,N-Dimethyl-α-naphthylamine and sulfanilic acid

Question 43

Differentiates Enterobacteriaceae (oxidase-negative) from Plesiomonas (oxidase-positive)

Answer 43

oxidase test

Question 44

reagent in oxidase test

Answer 44

tetramethyl-p-phenylenediamine dihydrochloride

Question 45

positive reaction of oxidase test

Answer 45

purple or lavender color

Question 46

enzyme in oxidase test

Answer 46

cytochrome C oxidase

Question 47

Determines whether a microorganism can hydrolyze urea

Answer 47

urease test

Question 48

enzyme in urease test

Answer 48

urease enzyme

Question 49

indicator in urease test

Answer 49

phenol red

Question 50

positive reaction in urease test

Answer 50

bright pink colorr

Question 51

It is commonly used to distinguish the genus proteus from other enteric bacteria.

Answer 51

urease test

Question 52

positive result in gelatin hydrolysis test

Answer 52

Liquefaction

Question 53

perecent of agar in motility test medium

Answer 53

0.4%

Question 54

positive reaction in motility test

Answer 54

movement away the stab line or hazy appearance throughout the medium

Question 55

routine medium used in motility test

Answer 55

SIM (Sulfide-Indole-Motility) Agar