Immunophenotyping Flashcards

(34 cards)

0
Q

Myeloid differentiation

A

First to appear is cD33, then cd 13, 14,117

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1
Q

Uncommitted hematopoietic progenitor cells

A

CD34+

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2
Q

Erythroid maturation

A

Appearance of cd 71 and cd 235a, loss of cd 33

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3
Q

Megakaryocyte differentiation

A

Cd 41 (glycoprotein IIb) and cd 61 (glycoprotein 2 b-3a)

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4
Q

NK cell differentiation

A

CD 56, 57

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5
Q

Monocyte differentiation

A

Cd 11, 14, 15, 64

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6
Q

TdT and HLA DR

A

Expressed on b and t lymphocytes

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7
Q

B cell maturation

A

Cd 10, 19, 20

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8
Q

T cell maturation

A

Cd 3, 4, 7, 8

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9
Q

CLL

A

CD 20, 5, 23

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10
Q

Mantle cell

A

CD 5, 19, 20, fmc7 positive
CD 23 NEGATIVE
t (11;14) Cyclin d 1

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11
Q

Marginal zone lymphoma

A

CD 19, 20, 43 positive

CD 5, 10 Negative

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12
Q

Classic Hodgkin lymphoma

A

CD 15, 30 positive

CD 20, 45 negative

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13
Q

Nodular lymphocyte predominant Hodgkin lymphoma

A

CD 15, 30 NEGATIVE

CD 20, 45 Positive

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14
Q

Follicular lymphoma

A

CD 10, 19, 20 positive
CD 5, 24 negative
t (14;18), bcl2

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15
Q

Hairy Cell leukemia

A

Cd 20, 35, 103 Positive

If pancytopenia and cd 103 positive, think hairy cell

16
Q

Lymphoplasmacytic lymphoma

A

CD 20, 38, 138 positive with light chain restricted plasma cells

17
Q

Multiple myeloma

A

Cd 20 NEGATIVE

CD 56, 38, 138 positive with light chain restricted plasma cells

18
Q

Acute B cell lymphoblastic leukemia

A

TdT positive
Cd 10, 19, 20, 22, 45, 79a Positive
Cd 3 negative

19
Q

Precursor B cell ALL

A

Cd 10, 19, 34 positive

20
Q

Mature B ALL (burkitts)

A

CD 10, 19, 20, 22, 25 and surface immunoglobulin positive

21
Q

T cell prolymphocytic leukemia (PLL)

A

CD 3, 7 56 positive

22
Q

T cell large granular leukemia

A

Cd 3, 7, 8, 16 positive

23
Q

NK leukemia

A

Cd 7, 56 positive

24
Peripheral T cell lymphoma
Cd 3, 4, 4, 7, 30 positive
25
Angioimmunoblastic lymphoma
Cd 3, 5, 7, 56 positive | Cd 30 negative
26
Anaplastic large T cell lymphoma
Cd 3, 5 and 7 +/-, cd 4 +, cd 30 +, cd 56 negative t(2;5) ALK ALK positive has better prognosis than negative
27
Bernard soulier syndrome
``` LARGE platelets Normal pt ptt Prolonged bleeding time Decrease in GP1b receptor ABNORMAL AGGREGATION TO RISTOCETIN, but normal to collagen and adp ```
28
Glanzmans thrombaathenia
``` NORMAL appearing platelets Normal pt ptt vwf Prolonged bleeding time Decrease in GP2b3a receptor ABNORMAL AGGREGATION TO Collagen and adp, but normal to ristocetin ```
29
Congenital afibrinogenemia
Same platelet aggregation as glanzmans but ptt ptand thrombin time prolonged
30
Grey platelet syndrome
Alpha granule deficiency | Abnormal aggregation to collagen
31
Dense granule deficiency
Puerto Rican albinism HSP1 gene Low platelet ADP Second wave of aggregation in response to ADP and epinephrine absent
32
Quebec platelet disorder
Normal coags Abnormal aggregation with epinephrine Due to increased urokinase plasminogen activator
33
Chediak higashi syndrome
Albinism which low IQ, nystagmus and hepatosplenomegaly Pancytopenia LYST gene defect Lymphohistiocytic infiltration of all organ systems