Immunophenotyping Flashcards
(34 cards)
Myeloid differentiation
First to appear is cD33, then cd 13, 14,117
Uncommitted hematopoietic progenitor cells
CD34+
Erythroid maturation
Appearance of cd 71 and cd 235a, loss of cd 33
Megakaryocyte differentiation
Cd 41 (glycoprotein IIb) and cd 61 (glycoprotein 2 b-3a)
NK cell differentiation
CD 56, 57
Monocyte differentiation
Cd 11, 14, 15, 64
TdT and HLA DR
Expressed on b and t lymphocytes
B cell maturation
Cd 10, 19, 20
T cell maturation
Cd 3, 4, 7, 8
CLL
CD 20, 5, 23
Mantle cell
CD 5, 19, 20, fmc7 positive
CD 23 NEGATIVE
t (11;14) Cyclin d 1
Marginal zone lymphoma
CD 19, 20, 43 positive
CD 5, 10 Negative
Classic Hodgkin lymphoma
CD 15, 30 positive
CD 20, 45 negative
Nodular lymphocyte predominant Hodgkin lymphoma
CD 15, 30 NEGATIVE
CD 20, 45 Positive
Follicular lymphoma
CD 10, 19, 20 positive
CD 5, 24 negative
t (14;18), bcl2
Hairy Cell leukemia
Cd 20, 35, 103 Positive
If pancytopenia and cd 103 positive, think hairy cell
Lymphoplasmacytic lymphoma
CD 20, 38, 138 positive with light chain restricted plasma cells
Multiple myeloma
Cd 20 NEGATIVE
CD 56, 38, 138 positive with light chain restricted plasma cells
Acute B cell lymphoblastic leukemia
TdT positive
Cd 10, 19, 20, 22, 45, 79a Positive
Cd 3 negative
Precursor B cell ALL
Cd 10, 19, 34 positive
Mature B ALL (burkitts)
CD 10, 19, 20, 22, 25 and surface immunoglobulin positive
T cell prolymphocytic leukemia (PLL)
CD 3, 7 56 positive
T cell large granular leukemia
Cd 3, 7, 8, 16 positive
NK leukemia
Cd 7, 56 positive