Indirect ELISA Flashcards
(4 cards)
1
Q
Solid surface
A
Ags passively adsorb in to this surface
Bottom of each well
Binding, blocking, Ab reaction all 3 occur in solid surface
The well of polystyrene microplate
2
Q
Primary antibody
A
Detect the target. In liquid sample.
3
Q
Secondary antibody
A
This is linked with enzyme. And bind with primary antibody
4
Q
Label
A
Enzyme tag on secondary antibody and creating a measurable signal
