Into To Light Microscope

Question 1

Why are students each given an assigned microscope?

Answer 1

Some students work faster than others

Question 2

What should you do if there is a problem with your microscope upon initial examination?

Answer 2

Inform the instructor

Question 3

How should microscopes by carried?

Answer 3

With two hands, one one the arm of the microscope and the other on the base.

Question 4

What things should you check for when first inspecting your microscope?

Answer 4

Check to see if the microscope was put away properly by the last user. Look for oil on stage and/or objective.

Question 5

What is used to clean microscope lenses?

Answer 5

Lens paper

Question 6

What is a parfocal lens?

Answer 6

Lens that stays in focus when magnification/focal length is changed

Question 7

At what objective should you utilize the coarse objective knob?

Answer 7

Scanning objective

Question 8

Why is the 100X objective also called the oil immersion objective?

Answer 8

It uses immersion oil in order to get good resolution or clarity

Question 9

What happens if oil is not removed from the objective after use?

Answer 9

It can make a mess and ruin the objective lens

Question 10

Which microscope features allow you to manipulate the amount of light hitting the image or specimen?

Answer 10

Iris diaphragm and condense focus knob

Question 11

Is more light always better to view a specimen?

Answer 11

Not always

Question 12

What steps should be taken to clean up the microscope before being out away?

Answer 12

1) clean the lenses and stage
2) center the clip on the stage and rotate the objectives so the 4X is in a position
3) wrap cord correctly around the base and place the cover over it
4) make sure it goes in the correct spot

Question 13

A compound light microscopes uses light transmitted _________

Answer 13

Through

Question 14

What characteristics must Specimens

have to be seen with a compound light microscope?

Answer 14

They appear solid black

Question 15

Why are stains used on some microscopic organisms, such as bacteria?

Answer 15

They give artificial color to the specimens and allow the observe to see them clearer

Question 16

What is the function of the condenser?

Answer 16

Takes weak scattered light and concentrates it into a condensed beam

Question 17

What is refractions?

Answer 17

Bending of light wave that are created by the photons themselves.

Question 18

What are the 2 key features of the compound light microscopes?

Answer 18

Magnification and resolution

Question 19

What is the ocular power of our microscopes?

Answer 19

10X

Question 20

How do you calculate the total power of magnification?

Answer 20

Multiply objective power by 10.

Question 21

What is resolution?

Answer 21

The ability to distinguish 2 points as being separate

Question 22

What factors affect the resolution of a microscope?

Answer 22

1) specimen characteristics
2) use of oils
3) type of light used
4) quantity of the microscope

Question 23

Smaller wavelengths of light result in __________

Answer 23

Greater potential resolution

Question 24

Would larger or smaller “balls” provide better resolution?

Answer 24

Smaller

Question 25

Why are compound light microscopes limited to a magnification of about 2,000x?

Answer 25

The image cannot be clearly resolved and the image looks more distorted as magnification increases