Into To Light Microscope Flashcards
(25 cards)
Why are students each given an assigned microscope?
Some students work faster than others
What should you do if there is a problem with your microscope upon initial examination?
Inform the instructor
How should microscopes by carried?
With two hands, one one the arm of the microscope and the other on the base.
What things should you check for when first inspecting your microscope?
Check to see if the microscope was put away properly by the last user. Look for oil on stage and/or objective.
What is used to clean microscope lenses?
Lens paper
What is a parfocal lens?
Lens that stays in focus when magnification/focal length is changed
At what objective should you utilize the coarse objective knob?
Scanning objective
Why is the 100X objective also called the oil immersion objective?
It uses immersion oil in order to get good resolution or clarity
What happens if oil is not removed from the objective after use?
It can make a mess and ruin the objective lens
Which microscope features allow you to manipulate the amount of light hitting the image or specimen?
Iris diaphragm and condense focus knob
Is more light always better to view a specimen?
Not always
What steps should be taken to clean up the microscope before being out away?
1) clean the lenses and stage
2) center the clip on the stage and rotate the objectives so the 4X is in a position
3) wrap cord correctly around the base and place the cover over it
4) make sure it goes in the correct spot
A compound light microscopes uses light transmitted _________
Through
What characteristics must Specimens
have to be seen with a compound light microscope?
They appear solid black
Why are stains used on some microscopic organisms, such as bacteria?
They give artificial color to the specimens and allow the observe to see them clearer
What is the function of the condenser?
Takes weak scattered light and concentrates it into a condensed beam
What is refractions?
Bending of light wave that are created by the photons themselves.
What are the 2 key features of the compound light microscopes?
Magnification and resolution
What is the ocular power of our microscopes?
10X
How do you calculate the total power of magnification?
Multiply objective power by 10.
What is resolution?
The ability to distinguish 2 points as being separate
What factors affect the resolution of a microscope?
1) specimen characteristics
2) use of oils
3) type of light used
4) quantity of the microscope
Smaller wavelengths of light result in __________
Greater potential resolution
Would larger or smaller “balls” provide better resolution?
Smaller
