Midterm: Intro to Lab

Question 1

What are the five I’s?

Answer 1

1) Inoculation
2) Incubation
3) Isolation
4) Inspection
5) Identification

Question 2

What is inoculation?

Answer 2

The implantation of microorganisms into or upon culture media

Question 3

What is incubation?

Answer 3

Placing microbes in the ideal temp range for optimal growth

Question 4

What is isolation?

Answer 4

The separation of microbes to create discrete individual colonies

Question 5

What is inspection?

Answer 5

The examination of media following inoculation snd incubation

Question 6

What is identification?

Answer 6

Utilizing specific lab techniques to determine the specific nature of a particular microbe or microbes

Question 7

What is a pure culture?

Answer 7

A culture that has only one species

Question 8

What does sterile mean?

Answer 8

Completely free of all life forms, including spores and viruses

Question 9

How do we achieve sterility in the lab?

Answer 9

All tools and Petri dishes are sterilized. Often involves an autoclave.