Intro To Histo Flashcards
Group of cells with interrelated functions
Tissue
Extracellular matrix composition
Fibers & Ground substance
-the study of tissues, their functions, and their arrangement to constitute an organ
-a.k.a Microscopic Anatomy
Histology
Enumerate the 8 Tissue processing steps
- Fixation
- Decalcification
- Dehydration
- Clearing
- Embedding
- Cutting & Sectioning
- Staining
- Mounting
Commonly used reagent for fixation
Formalin
Step to preserve the organ and terminate cell metabolism
Fixation
Step to soften samples
Decalcification
Commonly used reagent in decalcification
Nitric acid
Step that is done by successively bathing the specimen in mixture of ethanol and water from 70% to 100%
Removes water out of tissue
Dehydartion
Commonly used reagent in dehydration
Ethanol
Step for Removal of dehydrating agent by immersing the specimen in the solvent
Clearing
Commonly used reagent for Clearing
Xylene & Toluene
Step where the tissue is placed in a melting paraffin and where clearing reagent is evaporated
Embedding
Optimal temperature for paraffin wax to melt
52 - 60 degrees Celcius
-Step to trim appropriately sized blocks
-Removal of excess paraffin
Cutting & Sectioning
Tool used for cutting & sectioning
Microtome
Deparaffinization & Rehydration
Xylene - 5 mins
Xylene - 5 mins
Absolute Ethanol - 3 mins
Absolute Ethanol - 3 mins
95% Ethanol - 3 mins
95% Ethanol - 3 mins
Distilled water - 30s to 1 min
Step of application of color to the tissue to highlight structures
Staining
Commonly used reagent in Staining
Hematoxylin
Eosin
Stain for DNA
Feulgen reaction
Stain for carbohydrates
Periodic acid schiff
Stain for lipids
Sudan black
Stain for reticular fibers
Silver stain
Average tissue processing time in Ph Laboratories
48 to 72hrs
