Intro to Histology Flashcards
(14 cards)
What is the most common stain?
a) which stains for acidic structures like nuclei (basic dye)
b) which stains for proteins like cytoplasm (acidic dye)
1.Hematoxylin
A basic dye
Stains strictures containing acids (e.g. nuclei) blue (arrow)
- Eosin
An acidic dye
Stains structures containing protein (e.g cytoplasm) pink (square)
What is the following stain:
- Stains Bacteria
- background is yellow
- positive is PURPLE (peptidoglycan)
- Gram stain
What is the following stain:
Stains Fungi, Glycogen
- background is light pink
- positive is BRIGHT pink (magenta)
Periodic acid–Schiff (PAS)
What is the following stain:
–Stains fungi
- background green
- positive = BLACK
Grocott methenamine silver stain (GMS)
What is the following stain:
-Stains - Mycobacteria, Actinomyces (background blue / positive red)
Acid Fast
What stain is used for antibodies?
IHC
positive = BROWN/RED
State the following for skeletal, cardiac & smooth muscle
- Striations
- Nuclei
- Intercalated disks
SKELETAL:
- Yes striations
- Multiple Nuclei
- No disks
Cardiac:
- Yes striations
- Single Nuclei
- YES disks
SMOOTH:
- NO!! striations
- Single Nuclei
- No disks
______ tissue is made of fibroblasts with nuclei that are cigar shaped and evenly distributed.
CONNECTIVE tissue
The following describes what type of tissue:
- No defined boundary
- Linear Eosinophilic
- Indistinct cell border
- Uniform cytoplasm
- Cigar shaped nuclei
- Evenly distributed nuclei
CONNECTIVE TISSUE
The following describes what type of tissue:
- Fasciles w/ perineum
- WAVY Eosinophilic
- DISTINCT cell border
- Uniform cytoplasm
- FOAMY shaped nuclei
- nuclei in CENTER of cell
Nervous tissue
The following describes what type of tissue:
- Bundles w/ perimysium
- WAVY Eosinophilic
- DISTINCT cell border
- Uniform cytoplasm
- CIGAR shaped nuclei
- nuclei in CENTER of cell
Smooth Muscle
The following describes what type of tissue:
- Bundles w/ perimysium
- LINEAR Eosinophilic
- DISTINCT cell border
- STRIATED cytoplasm
- CIGAR shaped nuclei
- nuclei in periphery/center
SKELETAL/ cardiac muscle
Chronic inflammation lacks ______
NEUTROPHILS
____ are multi lobed nuclei
and ____ are bilobed
(eosinophilic / neutrophil)
- Neutrophil = multi
2. Eosinophil = bi
