Introduction

Question 1

Describe the COBS process.

Answer 1

Cesarean-originated barrier sustained.
Hysterectomy performed on near-term dam. Gravid uterus pulled through a disinfectant solution into a sterile flexible film isolator where pups were removed and suckled on axenic fosters. After being mated to expand their numbers and associated with a cocktail of nonpathogenic microflora to normalize their microflora, animals transferred to barrier room.

Question 2

Define incidence.

Answer 2

The rate of new contaminations

Question 3

Define prevalence.

Answer 3

The percentage positive within a time period.

Question 4

What highly environmentally stable and highly contagious enterotropic pathogens still cause disease outbreaks? Which pathogens are especially prominent in transgenic animals?

Answer 4

MPV, mouse rotavirus, MHV
Helicobacter, MNV, Pasteurella pneumotropica, and parasites

Question 5

Why are viruses especially targeted for removal?

Answer 5

As obligate intracellular organisms they are inherently invasive.

Question 6

Define SOPF.

Answer 6

Specific opportunistic pathogen free