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RUSVM Microanatomy I > Introduction > Flashcards

Flashcards in Introduction Deck (19)
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1
Q

The science which deals with structures of healthy body of animals and humans. The structure is revealed by naked eye observation and it is based on dissection.

A

Anatomy

2
Q

Encompasses all those structures accessible by dissection and direct inspection

A

Gross Anatomy

3
Q

The study of the tissues of the body and how cells and tissue integrate to form organs

A

Microanatomy

4
Q

The science which deals with ontogenetic development

A

Embryology

5
Q

List the 3 branches of histology

A

1) Cytology (deals with the structure and function of the cell)
2) General Histology (deals with structure of tissues)
3) Special Histology (deals with microscopic structure of organs)

6
Q

What are the two interacting components that make up tissues?

A

1) Cells

2) Extracellular matrix (ECM)

7
Q

What microscope allows observation of LIVING non-stained structures?

A

Phase contrast microscopy

8
Q

List some advantages of LM

A
  • relatively inexpensive
  • provides rapid diagnosis
  • allows observation of living specimens
9
Q

List some disadvantages of LM

A
  • image is 2-D
  • resolving power is limited by the wavelength of light
  • requires maintenance
  • requires expertise for proper diagnoses
10
Q

What type of microscope could you use to view a 3-D image such as an embryo?

A

Stereomicroscope aka Dissecting microscope

11
Q

List some advantages of dissecting scopes

A
  • relatively inexpensive
  • practical/versatile
  • 3-D image
  • can be used in microsurgery and with other types of specimens
12
Q

List some disadvantages of dissecting scopes

A
  • low resolving power

- needs to be maintained

13
Q

What are the two types of electron microscopy?

A

1) Transmission Electron Microscopy (TEM)

2) Scanning Electron Microscopy (SEM)

14
Q

List some advantages of TEM

A
  • Great resolving power

- very useful for rapid diagnosis of viruses and other microscopic organisms

15
Q

List some disadvantanges of TEM

A
  • Image is 2-D
  • Image is Black&White
  • Cannot be used in living object
  • Very expensive
16
Q

What specific conditions must a specimen meet in order to be observed under the common Bright field light microscope (LM) and/or TEM?

A

1) must be well preserved=retain structure and molecular composition
2) be sufficiently thin to allow light transmission
3) have enough contrast to observe details

17
Q

What are the steps in the tissue processing for observation in a microscope?

A
F (fat)- fixation
D (dogs)- dehydration
C (can)- clearing
I (indeed)-infiltration
E (eat)- embedding
18
Q

What substance is used during fixation?

A

10% buffered formalin

19
Q

What is used to cut tissues for slides?

A

Microtome (cuts 1-7 micrometers thick)