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Flashcards in Introduction Deck (19)
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1

The science which deals with structures of healthy body of animals and humans. The structure is revealed by naked eye observation and it is based on dissection.

Anatomy

2

Encompasses all those structures accessible by dissection and direct inspection

Gross Anatomy

3

The study of the tissues of the body and how cells and tissue integrate to form organs

Microanatomy

4

The science which deals with ontogenetic development

Embryology

5

List the 3 branches of histology

1) Cytology (deals with the structure and function of the cell)
2) General Histology (deals with structure of tissues)
3) Special Histology (deals with microscopic structure of organs)

6

What are the two interacting components that make up tissues?

1)Cells
2)Extracellular matrix (ECM)

7

What microscope allows observation of LIVING non-stained structures?

Phase contrast microscopy

8

List some advantages of LM

-relatively inexpensive
-provides rapid diagnosis
-allows observation of living specimens

9

List some disadvantages of LM

-image is 2-D
-resolving power is limited by the wavelength of light
-requires maintenance
-requires expertise for proper diagnoses

10

What type of microscope could you use to view a 3-D image such as an embryo?

Stereomicroscope aka Dissecting microscope

11

List some advantages of dissecting scopes

-relatively inexpensive
-practical/versatile
-3-D image
-can be used in microsurgery and with other types of specimens

12

List some disadvantages of dissecting scopes

-low resolving power
-needs to be maintained

13

What are the two types of electron microscopy?

1) Transmission Electron Microscopy (TEM)
2) Scanning Electron Microscopy (SEM)

14

List some advantages of TEM

-Great resolving power
-very useful for rapid diagnosis of viruses and other microscopic organisms

15

List some disadvantanges of TEM

-Image is 2-D
-Image is Black&White
-Cannot be used in living object
-Very expensive

16

What specific conditions must a specimen meet in order to be observed under the common Bright field light microscope (LM) and/or TEM?

1) must be well preserved=retain structure and molecular composition
2) be sufficiently thin to allow light transmission
3) have enough contrast to observe details

17

What are the steps in the tissue processing for observation in a microscope?

F (fat)- fixation
D (dogs)- dehydration
C (can)- clearing
I (indeed)-infiltration
E (eat)- embedding

18

What substance is used during fixation?

10% buffered formalin

19

What is used to cut tissues for slides?

Microtome (cuts 1-7 micrometers thick)