Introduction To Histology

Question 1

Deals with the physiologic structure and function of human cells and tissues. it is also known as microscopic anatomy or micro anatomy

Answer 1

Human histology

Question 2

Histology is the microscopic counterpart for gross anatomy where it focuses on larger structures visible without the use of a microscope

Answer 2

True

Question 3

Treatment of tissue with chemical

Answer 3

Fixation

Question 4

Removal of water from the tissue sample

Answer 4

Dehydration and clearing

Question 5

Infiltration of tissue sample with paraffin

Answer 5

Embedding

Question 6

Cutting tissue sample bisection into specific equal increments

Answer 6

Sectioning

Question 7

Placing the tissue sample on adhesive glass slides

Answer 7

Mounting and staining

Question 8

Fixation

Answer 8

10% formaldehyde solution uses:
-Preserves the tissues structure
-prevents enzymatic degradation of cells and tissues by autolysis
-kills pathogenic microorganisms
- hardens tissue for histopath exam

Question 9

Other fixatives

Answer 9

•Zenker’s - w/ mercuric chloride, for lm, excellent fixative
•Bouin’s- w/ picric acid, testicular biopsies
•Carnoy’s- w/ ethanol, chloroform & glacial acetic acid, dissolves fat

Question 10

Dehydration

Answer 10

Specimens dehydrated in a series of increasing alcohol solutions 70% 80% 95%

Question 11

Clearing

Answer 11

Removes the alcohol solution (xylene chloroform)

Question 12

Evens out the wax or paraffin embedded tissue using a sharp knife before sectioning is done

Answer 12

Trimming

Question 13

Commonly used stains

Answer 13

Hematoxylin
eosin
toluidine blue

Question 14

Forms the basophilic components of the cell

Answer 14

Hematoxolin

Question 15

Forms the acidophilic components of the cell

Answer 15

Eosin

Question 16

Forms the metachromic component of the cell

Answer 16

Toluidine blue

Question 17

Very good staining procedure for histology specimens for it displays the structural features of cells and tissues

Does not adequately reveal structural components of lipids basement membrane and reticular fibers

Answer 17

Hematoxylin and eosin

Question 19

Carries a net negative charge on its colored portion
Result: orange, red, dark pink

Answer 19

Acidic dye

Question 20

Stains under acidic dye

Answer 20

Acid fuschin
eosin
orange g

Question 21

Carries a net positive charge on its colored portion
Results: blue, purple, green

Answer 21

Basic dye

Question 23

Examples of basic dye stain

Answer 23

Methylene blue
toluidine blue
hematoxylin

Question 24

This is a special stains for glycogen, fungi and parasites

Answer 24

Periodic acid schiff (PAS)

Question 25

Acid fast org

Answer 25

Ziehl- Neelsen

Question 26

"fungi & pneumocystis"

Answer 26

Grocott's

Question 27

Fungi, amoeba, trichomonas

Answer 27

PAS

Question 28

Helicobacter etc.

Answer 28

Dieterle

Question 29

Neuroendocrine cells

Answer 29

Argentaffin stains

Question 30

Amyloid

Answer 30

Congo red stain

Question 31

Connective tissue & basement membrane

Answer 31

Reticulin stain

Question 32

Melanin

Answer 32

Fontanna masson

Question 34

Lipids

Answer 34

Oil red o

Question 35

Histopath procedure wherein instant pato disease is needed by surgeon

Answer 35

Frozen sections

Question 36

The specificity of a reaction between an antigen and an antibody - uses fluorescent dyes

Answer 36

Immunocytochemistry (IHC)

Question 37

Glycoproteins that are produced by specific cells of the immune system in response to a foreign protein

Answer 37

Antibody "immunoglobulins"

Question 38

Foreign protein at which antibody reacts

Answer 38

Antigen

Question 39

Are chemicals that absorb light of different wavelengths example uv light

Answer 39

Fluorescent dyes

Question 40

Method used in the study of cells and its structure using microscope

Answer 40

Microscopy

Question 41

Major kind of microscope that utilizes light for elimination

Answer 41

Light microscope

Question 42

Major kind of microscope uses an electron beam

Answer 42

Electron microscope

Question 43

H

Question 44

It's a part of a microscope in which the image formed by the objective lens may be directly examined

Answer 44

Ocular lens

Question 45

Gather the light that has passed through the specimen it has

Answer 45

Objective lens

Question 46

To focus the beam of light at the specimen

Answer 46

Condenser lens

Question 47

On which the slide is placed

Answer 47

Stage

Question 48

Controls the movement of the stage for proper focusing

Answer 48

Stage control

Question 49

Uses the fine power objective and course power objective

Answer 49

Focusing control

Question 50

Focuses the light being from the light source to the condenser

Answer 50

Field diaphragm

Question 51

For specimen illumination

Answer 51

Light source

Question 52

It is the ratio between the size of an image produced by the microscope and its actual size

Answer 52

Magnification

Question 53

A measure of clarity of an image

Answer 53

Resolution

Question 54

The ability to visualize a particular cell structure depending on how different it looks from an adjacent structure

Answer 54

Contrast