investigations

Question 1

how do you test temperature change produced by respiration

Answer 1

-put two beans in two seperate vacuum flaks
-make sure air is left in flasks for aerobic respiration
-put a thermometer in each and seal top with cotton wool
-record temp each day for a week

Question 2

how to test for co2 with an indicator

Answer 2

-soak benas in water for a day to germinate
-put in hydrogen carbonate indicator
-place a platform of gauze into each test tube and put beans on them
-seal with rubber bung
-leave for hour
-co2 produces will turn indicator orange to yellow

Question 3

investigate conditions needed for germination

Answer 3

have four boiling tubes with diff conditions have cotton wool at bottom of each
-tube1- control
-tube2- no water, o2, room temp
-tube3-water,02,low temp
tube4- water, no 02, room temp

Question 4

investigating respiration of yeast

Answer 4

-mix sugar, yeast, distiled water
-add oil for anaerobic conditions
-add bung leading to 2nd tube of water
-place tube with yeast into water bath
-leave tube to warm up and count how many bubbles in a minute
-repeat with diff temps

Question 5

rate of transpiration

Answer 5

-cut shoot at angle underwater
-assemble potomètre in water
-keep end of capillary tube underwater
-shut tap
-remove end of capillary tube till bubble forms
-record starting measurement with ruler
-leave for an hour and record distance traveled

Question 6

rate of photosynthesis

Answer 6

-put Canadian pondweed into test tube with water sealed with a bung connected to a syringe connected to water in a cappilarory tube
-put light source at specific distance
-02 will collect in capillary tube
-measure length of bubble
-repeat with light source at diff distance

Question 7

energy released from food

Answer 7

-weigh some beans and put on skewer
-add a volume of water to a boiling tube
-measure temp of water
-set fire to beans with bunsen burner until it goes out
-relight bean until won’t relight
-measure temp of water after
-energy in food+ mass of water times temp change

Question 8

test for glucose

Answer 8

-add Benedict’s solution
-put in water bath
-food contains glucose go from blue to green to yellow to orange to brick red

Question 9

test for starch

Answer 9

-iodine
-will go from orange/ yellow to blue/black

Question 10

test for protein

Answer 10

add biuret solution
-shake solution
-go fro blue to lilac/purple

Question 11

test for lipids/fats

Answer 11

-add Sudan (III)
-shake
-will separate and if lipids/fats present top layer will go brick red

Question 12

how temp affects enzyme activity

Answer 12

-but catalase into test tube with hydrogen peroxide solution
-seal with bung attached a boiling tube in a water bath
-record how much o2 is produced
-repeat at diff temps