invitro diagnostics

Question 1

ELISA

Answer 1

enzyme-linked immunoassay

Question 2

enzymee-linked immunoassay

Answer 2

enzymes are used to analyse antibody and antigen interactions

Question 3

advantages of elisa

Answer 3

high specificity
quick and inexpensive
multiple sample analysis

Question 4

immunochromatography

Answer 4

the liquid in antigen test contains viral proteins
if antigen present it attaches to the anti antigen antibody

Question 5

hapten concept

Answer 5

makes non-immunogenic structures visible to immune system which helps with diagnosis and vaccine applications

Question 6

types of reporter enzymes

Answer 6

horseradish peroxidase
alkaline phosphatase
glucose oxidase
b-galacrosidase

Question 7

how do reporter enzymes work

Answer 7

they covalently link to antibodies/antigen allowing visual detection of immune complex

Question 8

competitive immunoassay

Answer 8

relies on competition between the antigen of interest and reference antigen

Question 9

non-competitive immunoassay

Answer 9

does not rely on the competition between the antigen of interest and reference antigen

Question 10

recombinant antigen

Answer 10

derived from e.coli yeast cells and mammalian cells