Irreversible inhibitors

Question 1

Irreversible inhibitors
types

Answer 1

1.Group Speific Covalent Modifying Agent
2.Affinity Labels
3.Transitional State Analogs
4. Suicidal substrate
5.Sucidal Inhibitors

Question 2

Group Speific Covalent Modifying Agent examples

Answer 2

1.DIFP- Diisopropyl fluro phosphate= serine
PMSF-Phenyl methyl sulphonyl fluride = serine
Sarin and Soman (Nerve gas)= serine
2.Cysteine = iodoacetate idoacetamide

Question 3

Affinity Labels examples

Answer 3

(TPCK); Tosyl Pheklyalanyl Chloromethyl Ketome.
binds to His in chymotrisprin

Question 4

Transitional State Analog examples

Answer 4

Pyrrole 2-carbonylate similar to proline
Inhibit proline racemase

Question 5

Suicidal substrate examples

Answer 5

Fluro uracil (5-Fu): thymidylate synthase

Acyclovir: DNA polymerare

Allopurinol : Xanthine Oxidase

Question 6

Sucidal Inhibitors example

Answer 6

Asprain: Cyclooxygenase
Pencillın: trans peptidase