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Natural Products > Isolation And Purification Techniques > Flashcards

Isolation And Purification Techniques Flashcards

1
Q

Fractionation techniques

A

Chemical method
Physical method

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2
Q

Physical methods of fractionation

A

Separation funnel method
Chromatographic techniques
Fractional distillation
Fractional crystallization
Sublimation

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3
Q

Mechanisms of separation in chromatography

A

Adsorption chromatography
Partition chromatography
Affinity chromatography
Ion exchange chromatography
Size exclusion chromatography

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4
Q

Chromatographic techniques

A

Paper chromatography
Thin layer chromatography
Column chromatography
Gas chromatography
High performance liquid chromatography

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5
Q

Difference of extraction and isolation

A

Extraction: process of moving one or more analytes from samples or matrix to a physically separate location for further processing and analysis. Has low purity

Isolation: separation technique in obtaining a purified compound.

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6
Q

Fractionation

A

Process of separation of plant extracts into various fractions

further segregates the fractions into portions

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Natural Products (2 decks)

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