K101 Final Exam Practice Unit 5 Flashcards
(122 cards)
Ch 20: By fusing protein-coding eukaryotic DNA with bacterial promoter sequences, scientists can ensure:
A. that bacterial DNA sequences will be inserted into the eukaryotic DNA sequence.
B. that the eukaryotic DNA can be expressed in bacterial cells.
C. that no mutations will occur within the eukaryotic sequence.
D. that transcription of the eukaryotic DNA will not occur.
E. that introns of the eukaryotic DNA will not be expressed.
B. that the eukaryotic DNA can be expressed in bacterial cells.
Ch 20: Which of the recombinant DNA drugs is incorrectly matched with its indication (the disease it is prescribed to treat)?
A. Humulin (Lilly): Diabetes (Insulin)
B. Epogen (Amgen): Anemia (Erythropoetin)
C. Neupogen (Amgen): Multiple sclerosis (interferon)
D. Activase/TNKase (Genentech): AMI - Acure myocardial infarction (heart attack)
E. Regranex (Novartis): Diabetic foot ulcers (PDGF)
C. Neupogen (Amgen): Multiple sclerosis (interferon)
Ch 20: What process or enzyme was used for Step 1 at right?
A. PCR
B. a bacteriophage
C. a reverse transcriptase
D. a DNA ligase
E. a restriction enzyme
E. a restriction enzyme
Ch 20: In the polymerase chain reaction (PCR) technique, a heating phase and a cooling phase alternate in cycles. An original sample of DNA would have to pass through how many total cycles to amplify the DNA ~1 billion times? (What about 2 billion times? What about 1 trillion times?)
A. 20
B. 21
C. 30
D. 31
E. 40
C. 30
Ch 20: Which enzyme was used to produce the molecule at right?
A. ligase
B. transcriptase
C. a restriction enzyme
D. RNA polymerase
E. DNA polymerase
C. a restriction enzyme
Ch 20: Match the mAb (monoclonal Antibody) drug with the disease it is intended to treat: Herceptin
A. RSV (Respiratory syncytial Virus
B. Cancer (anti-VEGF, anti angiogenesis)
C. HER-2+ breast cancer
D. CD20+ Non-Hodgkins Lymphoma
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
C. HER-2+ breast cancer
Ch 20: Match the mAb (monoclonal Antibody) drug with the disease it is intended to treat: Rituxin
A. RSV (Respiratory syncytial Virus
B. Cancer (anti-VEGF, anti angiogenesis)
C. HER-2+ breast cancer
D. CD20+ Non-Hodgkins Lymphoma
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
D. CD20+ Non-Hodgkins Lymphoma
Ch 20: Match the mAb (monoclonal Antibody) drug with the disease it is intended to treat: Enbrel
A. RSV (Respiratory syncytial Virus
B. Cancer (anti-VEGF, anti angiogenesis)
C. HER-2+ breast cancer
D. CD20+ Non-Hodgkins Lymphoma
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
Ch 20: Match the mAb (monoclonal Antibody) drug with the disease it is intended to treat: Synagis
A. RSV (Respiratory syncytial Virus
B. Cancer (anti-VEGF, anti angiogenesis)
C. HER-2+ breast cancer
D. CD20+ Non-Hodgkins Lymphoma
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
A. RSV (Respiratory syncytial Virus
Ch 20: Match the mAb (monoclonal Antibody) drug with the disease it is intended to treat: Avastin
A. RSV (Respiratory syncytial Virus
B. Cancer (anti-VEGF, anti angiogenesis)
C. HER-2+ breast cancer
D. CD20+ Non-Hodgkins Lymphoma
E. T-cell autoimmune diseases like Rheumatoid arthritis and Psoriasis
B. Cancer (anti-VEGF, anti angiogenesis)
Ch 20: Gene Therapy treatments with new drugs like Kymriah and Yescarta (select all that apply)
A. Provide a safer bone marrow transplant procedure using matched donors
B. Are the first FDA-approved drugs for personalized immunotherapy to treat specific leukemias
C. Involve the use of a genetically engineered Chimeric Antigen Receptor T-cell to recognize proteins like CD19 on cancer cells
D. Require the formation of a blastocyst that is then used for gene editing
e. Can cure autoimmune disprders like diabetes and nultiple sclerosis
B. Are the first FDA-approved drugs for personalized immunotherapy to treat specific leukemias
C. Involve the use of a genetically engineered Chimeric Antigen Receptor T-cell to recognize proteins like CD19 on cancer cells
Ch 20: .Match the following compoments of the CRISPR Cas9 Gene Editing system to their function: Cas9
A. An endonuclease that can create double strand breaks in DNA
B. An RNA molecule engineered to be complementary to the target to be edited
C. A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
A. An endonuclease that can create double strand breaks in DNA
Ch 20: .Match the following compoments of the CRISPR Cas9 Gene Editing system to their function: sgRNA
A. An endonuclease that can create double strand breaks in DNA
B. An RNA molecule engineered to be complementary to the target to be edited
C. A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
B. An RNA molecule engineered to be complementary to the target to be edited
Ch 20: .Match the following compoments of the CRISPR Cas9 Gene Editing system to their function: PAM
A. An endonuclease that can create double strand breaks in DNA
B. An RNA molecule engineered to be complementary to the target to be edited
C. A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
C. A 3-6 nucleotide DNA sequence immediately downstream of the target site in the genomic DNA to be edited
Ch 20: Which of these statements is NOT TRUE regarding the procedure for making cloned animals by somatic cell nuclear transfer?
A. The recipient egg is e-nucleated before the procedure
B. The donor nucleus can be either from a male or a female cell
C. The donor nucleus is from a haploid, quiescent cell
D. A brief electric shock is used to simulate fertilization
E. A surrogate mother is used to carry the pregnancy to term
C. The donor nucleus is from a haploid, quiescent cell
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Ian Wilmut
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
D. cloning of dolly the sheep
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Jennifer Duodna
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
G. CRISPR Cas9
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: W. French Anderson
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
A first human gene therapy
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Shinya Yamanaka
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
j. induced pleuripotent stem cells
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Stanley Cohen
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Kary Mullis
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
I. polymerase chain reaction
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Herb Boyer
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
H. isolation of restriction enzymes, founder of Genentech
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: James Thompson
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
E. derivation of human embryonic cells
Ch 20: Where would Biotech be without these scientists? Briefly describe the contribution (experiment, technique) of these scientists in the timeline of Biotechnology::: Fred Sanger
A. first human gene therapy
B. dideoxy DNA sequencing
C. isolation of bacterial plasmids
D. cloning of dolly the sheep
E. derivation of human embryonic cells
F. whole genome shotgun sequencing
G. CRISPR Cas9
H. isolation of restriction enzymes, founder of Genentech
I. polymerase chain reaction
j. induced pleuripotent stem cells
B. dideoxy DNA sequencing