Key ID for Granulocyte Series

Question 1

What are the key IDs for Myeloblasts?

Answer 1

Myeloblasts:
• Large cell, 15-20u
• Round to oval nucleus
• High nucleus/cytoplasm ratio (4:1)
• Basophilic cytoplasm
• Absent or few granules
• Fine chromatin with or without nucleoli

Question 2

What are the key IDs of a promyelocyte?

Answer 2

Promyelocyte
• Presence of primary granules (red to purple) overlying the nucleus.
• No secondary granules.
• Round to oval nucleus.
• Likely larger than myeloblast

Question 3

What are the key IDs for a myelocyte?

Answer 3

Myelocyte
• Appearance of secondary granules.
• Smaller round to oval nucleus (may have one flattened side) & smaller nucleus to cytoplasm (2:1) ratio.
• Nucleoli no longer visible.
• Slight or no basophilia

Question 4

What are the key IDs for a metamyelocyte?

Answer 4

Metamyelocyte:
• Indented, kidney shaped nucleus.
• Indentation of nucleus <50% of the width of the nucleus.

Question 5

What are the key IDs for a band neutrophil?

Answer 5

Band Neutrophil:
• Indentation of nucleus >50% the width of the nucleus.
• Chromatin must be visible in thinnest section of nucleus.

Question 6

What are the key IDs for a segmented neutrophil?

Answer 6

Segmented Neutrophil:
Nucleus has 2-5 lobes connected by a thin filament without visible chromatin.