Kirby bauer test

Question 1

purpose of AST

Answer 1

To test the effectiveness of antibiotics
To give information to physicians on the proper antibiotics that will give to the
patient

Question 2

most common diffusion method of AST

Answer 2

Kirby- Bauer Diffusion Method

Question 3

diffusion method of AST where in antibiotics is in liquid form

Answer 3

Agar Cup Diffusion Method

Question 4

diffusion method of AST where in metal cylinder is incorporated and there, liquid antibiotic will be added

Answer 4

Agar Cylinder Diffusion Method

Question 5

antibiotics in strips

Answer 5

Gradient Diffusion Method

Question 6

2 types of dilution method

Answer 6

micro dilution
macro dilution

Question 7

Zone of inhibition is being measured using

Answer 7

vernier caliper

Question 8

Standardized Pure Inoculum

Answer 8

0.5 mcfarland

Question 9

It has a calibration, with a green light indicating that a 0.5 suspension is
already achieved. If the light is red, we will add demineralized water or NSS

Answer 9

Densitometer

Question 10

Mueller Hinton Agar pH

Answer 10

7.2 to 7.4

Question 11

Mueller Hinton Agar depth

Answer 11

5 mm

Question 12

Staphylococcus spp. & MRSA

Answer 12

MHA + 5% Sodium chloride

Question 13

Haemophilus spp., Streptococci spp.

Answer 13

MHA + 5% defibrinated blood sheep

Question 14

Proper spacing in MHA

Answer 14

18 mm or more than 15mm

Question 15

number of disk in big MHA (150mm)

Answer 15

12

Question 16

number of disk in small MHA (100mm)

Answer 16

6

Question 17

Indicates an organism may require a higher dose of antibiotic for longer period of time to be inhibited

Answer 17

Intermediate

Question 18

Indicates an organism is inhibited by recommended dose

Answer 18

Susceptible

Question 19

Indicates an organism is not inhibited by recommended dose

Answer 19

Resistance

Question 20

Zone of inhibition is reported in

Answer 20

millimeters (mm)

Question 21

For microorganisms with unpredictable susceptibilities, determine what is the lowest concentration of antibiotic
in which the bacteria will be inhibited.

Answer 21

Minumum Inhibitory Concentration

Question 22

Set up for Macrobroth/ Tube Dilution method

Answer 22

Mueller Hinton broth + Antibiotics + Inoculums of uniform volume.

Question 23

Set up for alamar system

Answer 23

Mueller Hinton broth + organism + Alamar blue indicator

Question 24

This system uses a traythat would contain the antibiotics

Answer 24

Alamar System

Question 25

color indicating growth in Alamar system

Answer 25

pink

Question 26

Uses automated multi-pronged inoculators calibrated to deliver the precise
volume of inoculum to each well

Answer 26

Microdilution

Question 27

Microdilution storage requirement

Answer 27

• 20 degrees celsius