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Bacteriology Finals > Water microbiology > Flashcards

Water microbiology Flashcards

1
Q

International Standard reference and has procedures intended for the examination of waters of a wide range of quality

A

Standard Methods for the Examination of Water and Wastewater

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2
Q

Administrative Order _____ Safe Water Drinking Act

A

Administrative Order 2017-0010

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3
Q

Parameter tested in water analysis

A

physical, chemical, microbiological, radiological

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4
Q

Drinking-water supplies should be free from contamination by human and animal excreta, which can contain a variety of microbial contaminants

A

Philippine National Standards for Drinking Water

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5
Q

Provide evidence of fecal contamination from
humans or warm-blooded animals

A

Indicator organism

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6
Q

True or False
“No organism fulfills all the criteria for an indicator organism, but the coliform
bacteria fulfill most.”

A

True

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7
Q

True or False
Ratio of indicator/pathogen should be high, there should be more pathogens than that of the indicator.

A

False; more indicator

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8
Q

The coliform group consists of several genera of bacteria belonging to the
family of _____

A

Enterobacteriaceae

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9
Q

Described as all facultative anaerobic gram-negative, non-spore-forming,
rod-shaped bacteria that ferment lactose with gas and acid formation
within 48 hours at 35 degrees C (+/- 2 degrees C).

A

Coliform bacteria

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10
Q

Describes a broad group of bacteria that include pathogens,
nonpathogens and opportunistic microorganisms

A

Heterotrophic Bacteria

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11
Q

Heterotrophic Plate Count procedures

A

Pour Plate
Spread Plate
Membrane filtration technique

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12
Q

Coliform Count procedures

A

Multiple tube fermentation technique
Membrane filtration technique
Enzyme-substrate test

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13
Q

Collection Container

A

Non-reactive borosilicate glass or plastic bottles

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14
Q

the required minimum amount of water sample

A

150mL

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15
Q

The containers must be able to contain at least _____ with additional space to facilitate mixing

A

250 ml

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16
Q

Sterilization requirements

A

121 degrees C for 15 mins and 15psi

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17
Q

A reducing agent is added to collection containers of water having residual chlorine or other halogens

A

Dechlorination

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18
Q

Neutralizes any residual halogen and prevents continuation of bactericidal action during sample transit

A

Sodium thiosulfate

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19
Q

Hold samples at a temperature _____ during transit

A

2C to 4C

20
Q

retention time in lab if NEGATIVE

A

3 days

21
Q

retention time in lab if POSITIVE

A

1 month

22
Q

Holding time for coliform count

A

30 hours

23
Q

Holding time for heterotrophic count

A

8 hours

24
Q

HPC procedure were in colonies are on the agar surface where they can be distinguished readily from particles and bubbles. it causes no heat shock but is limited to a small amount of sample volume 0.1 to 0.5ml

A

Spread Plate Method

25
Q

HPC procedure were in it is simple to perform and can accommodate volumes of sample or diluted
sample ranging from 0.1 to 2.0mL

A

Pour plate method

26
Q

HPC procedure were in it permits testing large volumes of low-turbidity water and is the method of
choice for low-count water (<1 – 10 CFU/mL)
→ Usually up to 100mL for those sites that usually sterile

A

membrane Filtration Method

27
Q

Limitations for membrane Filtration Method

A

adds membrane filter and has smaller display area

28
Q

tube-dilution method using a nutrient rich medium which detects and estimates the coliforms present in water

A

Multiple Tube Fermentation Technique

29
Q

Results in Multiple Tube Fermentation Technique are reported by ______

A

Most Probable Number

30
Q

Phases in Multiple tube fermentation

A

presumptive, confirmed, completed

31
Q

Broth used in presumptive phase

A

lauryl tryptose broth

32
Q

Broth used for confirmed phase

A

Brilliant green lactose bile broth

33
Q

Broth used for completed phase

A

Escherichia coli Broth

34
Q

Acid formation is not usually seen in this phase

A

confirmed phase

35
Q

MPN Reporting
5 positive tubes

A

> 8.0 mpn/100 ml

36
Q

MPN Reporting
4 positive tubes

A

8.0 mpn/100 ml

37
Q

MPN Reporting
3 positive tubes

A

4.6 mpn/100ml

38
Q

MPN Reporting
2 positive tubes

A

2.6 mpn/100ml

39
Q

MPN Reporting
1 positive tubes

A

1.1 mpn/100 ml

40
Q

MPN Reporting
0 positive tubes

A

<1.1 mpn/100 ml

41
Q

Uses hydrolysable substrate for the simultaneous detection of total coliform bacteria and Escherichia coli enzyme.

A

enzyme substrate test

42
Q

cleave chromogenic substrate

A

β-D-galactosidase

43
Q

cleaves the fluorogenic substrate

A

β-glucuronidase

44
Q

Standard sample volume of membrane filter technique

A

100 ml

45
Q

advantages of membrane filter technique

A

highly reproducible, more rapid result, larger sample volume, use to monitor variety or natural waters

Bacteriology Finals (7 decks)

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