Flashcards in KJB - Transcriptomics Deck (22):
What is an -ome?
A suffix that is attached to biological entities for describing very large-scale data collection and analysis.
All of the proteins produced by a cell at a given point in time.
What does transcriptomics and proteomics entail?
Involves looking at RNA and proteins produced by a given set of cells under a given set of conditions.
What is a gene expression profile?
It looks at all of your genes (20,000 - 30,000) at the same times and tells you which are on and off. If they are on, they will produce RNA + protein. If they are switched off, they will not. You can tell how much a gene is switched on by how much RNA and how many proteins are made. Some genes need to produce more product depending on what they are used for.
Why use gene expression profiling and how is it done?
To help characterise complex diseases.
1. Collect tissue samples from healthy and diseased participants.
2. Determine gene expression profiles.
3. Identify genes that are expressed differently in.
4. Use this information to:
- develop diagnostic tests
- identify new drug targets
What is a transcript?
An RNA molecule?
Which is bigger, RNA or DNA?
Which is more water soluble, RNA or DNA?
Which is very unstable and degrades quickly, RNA or DNA?
What techniques allow you to identify new drug targets?
Microarray gene chips, Spotted arrays and Affymetrix gene chips.
What is the basic principle of micro array gene chips?
Nucleic acid hybridisation
Microarrays are a solid support which contain...
... short pieces of single stranded DNA (probes) attached to it.
A single strand of DNA or RNA can pair specifically with a second strand of complementary nucleotide sequence via which bond?
The probes are...? What is added for it to pair?
Single stranded DNA. We add RNA sequence for it to pair.
Spotted arrays are also known as...
... two channel microarrays
The conditions to make comparisons between healthy and diseased samples using spotted arrays are:
1. You must let them stick to the array at the same time
2. You can only make comparisons between two samples on the SAME chip.
Do spotted arrays look at the whole set of genes?
NO. Only looks at a limited number of genes.
How are Affymetrix gene chips synthesised?
Photolithographically. Robots synthesis probes directly onto the glass support of the chip.
Difference between comparison making with spotted arrays and affymetrix gene chips?
Spotted arrays - can only make comparisons on the SAME chip.
Affymetrix gene chips - can make comparisons BETWEEN chips.
Difference in how Affymetrix gene chips (Photolithographic oligo chips) and spotted arrays express intensity.
Affymetrix chips - BRIGHTER chips are more strongly expressed.
Spotted arrays - BIGGER chips are more strongly expressed.
What are the 5 current applications of microarray technology?
1. Identifying new drug targets
2. Testing cellular activity of new drug molecules
3. Establishing toxicity profiles and identifying metabolic pathways of new drugs
4. Improving/ early diagnosis of conditions
5. Matching optimised drug treatment regimes to patient/tumour genotypes (pharmacogenomics)