KLUBSY: LABORATORY SAFETY Flashcards
(98 cards)
1
Q
Used to remove particles that may be harmful to the employee who is working with potentially infectious biologic specimens
A
Biosafety cabinets (BSCs)
2
Q
This must be used when performing procedures that generate
infectious aerosols
A
BSC
3
Q
For BSC Class I, what is its face velocity?
A
75
4
Q
for BSC Class II, A1, what is its face velocity?
A
75
5
Q
For BSC Class II, A2, what is its face velocity?
A
100
6
Q
For BSC Class II, B2, what is its face velocity?
A
100
7
Q
For BSC Class II B2, what is its face velocity?
A
100
8
Q
In at front
through HЕРА
to the outside
or into the
room through
HEPA. What BSC Class is this?
A
BSC Class I
9
Q
What is the airflow pattern for BSC Class II, A1?
A
R - 70%
E - 30%
*R for recirculated and E for exhausted
10
Q
What is the airflow pattern for BSC Class II, A2?
A
R = 70%
E = 30%
11
Q
What is the airflow pattern for BSC Class II, B1?
A
R = 30%
E = 70%
12
Q
What is the airflow pattern of BSC Class II, B2?
A
No recirculation,
total exhaust
to outside. Therefore,
R = 0%
E = 100%
13
Q
This BSC Class only exhausted air is filtered by HEPA filter
A
Class I
14
Q
In this BSC Class, air entering and air exhausted is filtered
A
Class II
15
Q
In this BSC Class, gloves are attached
A
Class III
16
Q
These are required to contain and expel noxious and hazardous
fumes from chemical reagents
A
chemical fume hoods
17
Q
used when working with some highly toxic and volatile
chemical
A
chemical fume hoods
18
Q
a partially enclosed ventilated work space for volatile
chemicals
A
chemical fume hoods
19
Q
T/F: Chemical fume hoods are used for storage of hazardous chemicals
A
FALSE.
NOT used
20
Q
Chemical fume hoods Periodically evaluate what?
A
Periodically evaluate ventilation
21
Q
Chemical fume hoods periodically evaluate ventilation by what?
A
by measuring face velocity
22
Q
Chemical fume hoods periodically evaluate ventilation by measuring face velocity using a what?
A
with a calibrated velocity meter
23
Q
Specialized clothing or equipment worn by an employee
for protection
A
Personnel protective equipment (PPE)
24
Q
These PPE protect the hands and arms
when using caustic chemicals.
A
gloves and rubberized sleeves
25
Equipment for Face and Eye protection?
goggles and face shield
26
What does HEPA mean?
High-efficiency particulate air
27
What is donning?
putting ON of PPE
*dONning
28
what is doffing?
Putting off of PPE
*dOFFing
29
What is the correct sequence of donning PPE?
Gown > masks/respirator > goggles/face shield > gloves
30
What is the correct sequence for doffing PPE?
Gloves > goggles/face shield > gown > mask/respirator > perform HANDWASHING
31
Single most important method to reduce infection
transmission
Handwashing
32
what must be utilized in proper handwashing?
Use soap and warm water
33
scrubbing time for handwashing?
20 seconds
34
what is an IMPORTANT step in handwashing?
mechanical friction
35
4 types of BIOLOGIC SAFETY
1-universal precaution
2-body substance isolation
3- standard precautions
4-transmission based precautions
36
Apply to blood and to other body fluids containing visible
blood.
Universal precaution
37
Recommend the use of PPE, handwashing after removal
of gloves, for handling and disposal of needles and other
sharps
Universal precaution
38
Emphasized the avoidance of contact with potentially
infectious, moist body fluid (except sweat) regardless of
the presence or absence of blood
Body substance isolation
39
Handwashing after glove removal not required unless
hands visibly soiled
Body substance isolation
40
Applies to all body fluids, secretions, excretions, (except sweat) and tissue specimens
standard precautions
41
Applies to human-based standards or calibrators, quality
control materials, and proficiency testing materials
standard precautions
42
Applies to non-intact skin and mucous membranes
standard precautions
43
what are the 3 categories of transmission based precautions?
-airborne
-droplet
-contact
44
this biological safety type has 3 categories: airborne, droplet, contact
transmission-based precautions
45
Used when SP (standard precaution) alone are insufficient
transmission based precautions
46
Used when for patients with known or suspected infection
transmission-based precautions
47
Required by OSHA to minimize the risk of exposure to
bloodborne pathogens
exposure control plan
48
the exposure control plan is required by?
OSHA (Occupational Safety and Health Administration)
49
Ensures protective safety of laboratory staff to potential
infectious bloodborne pathogen
Exposure control plan
50
Process that eliminates many or all pathogenic
microorganisms, except bacterial spores, on inanimate
objects
disinfection
51
Widely used disinfectant?
household bleach or sodium
hypochlorite
52
What is the ratio to make sodium hypochlorite disinfectant?
1 part bleach : 9 parts H2O
53
What are the 8 steps in case of a spill?
1-Alert others in area of the spill.
2-Wear appropriate protective equipment.
3-Use mechanical devices to pick up broken glass or other
sharp objects
4- Absorb the spill with paper towels, gauze pads, or tissue
5-Clean the spill site using a common aqueous detergent.
6-Disinfect the spill site using approved disinfectant or 10%
bleach, using appropriate contact time.
7-Rinse the spill with water
8-Dispose of all materials in appropriate biohazard
containers
*accdg to Turgeon, the 1st step in case of a spill is step #4.
54
A plan that provides safety policies, procedures & work
practices for all hazardous chemical
chemical hygiene plan
55
regulating and reducing exposure of lab personnel to
hazardous chemicals
chemical hygiene plan
56
this personnel must be designated for any lab
using hazardous chemicals
chemical hygiene officer
57
Informs a worker about the hazards of chemicals in the
workplace under normal conditions of use and
foreseeable emergencies
hazard communication standard
58
What does SDS mean?
safety data sheet
59
Requires that the chemical manufacturer, distributor,
or importer provides Safety Data Sheet (SDS) for each
HAZARDOUS chemical
hazard communication standard
60
what is the gold standard for chemical safety?
hazard communication standard
61
Recall:
which of the following is true for SDS?
HCS
62
what are the 6 information provided on every label of all chemicals?
1-product identifier
2-signal word
3-hazard statements
4-pictogram
5-precautionary statement
6-name, address, and phone number of supplier
63
GHS (Globally harmonized system Pictograms and Hazards:
Carcinogen
Health hazard
64
GHS (Globally harmonized system Pictograms and Hazards:
mutagenicity
health hazard
65
GHS (Globally harmonized system Pictograms and Hazards:
reproductive toxicity
health hazard
66
GHS (Globally harmonized system Pictograms and Hazards:
target organ toxicity
health hazard
67
GHS (Globally harmonized system Pictograms and Hazards:
aspiration toxicity
health hazard
68
GHS (Globally harmonized system Pictograms and Hazards:
flammable
flame
69
GHS (Globally harmonized system Pictograms and Hazards:
pyrophorics
flame
70
GHS (Globally harmonized system Pictograms and Hazards:
self-heating
flame
71
GHS (Globally harmonized system Pictograms and Hazards:
emits flammable gas
flame
72
GHS (Globally harmonized system Pictograms and Hazards:
self-reactives
flame
73
GHS (Globally harmonized system Pictograms and Hazards:
organic peroxides
flame
74
GHS (Globally harmonized system Pictograms and Hazards:
irritant (skin and eye)
exclamation mark
75
GHS (Globally harmonized system Pictograms and Hazards:
skin sensitizer
exlamation mark
76
acute toxicity (HARMFUL)
exclamation mark
77
GHS (Globally harmonized system Pictograms and Hazards:
narotic effects
exclamation mark
78
GHS (Globally harmonized system Pictograms and Hazards:
respiratory tract irritant
exclamation mark
79
GHS (Globally harmonized system Pictograms and Hazards:
hazardous to ozone layer (non-mandatory)
exclamation point
80
GHS (Globally harmonized system Pictograms and Hazards:
gases under pressure
gas cylinder
81
GHS (Globally harmonized system Pictograms and Hazards:
skin corrosion/burns
corrosion
82
GHS (Globally harmonized system Pictograms and Hazards:
eye damage
corrosion
83
GHS (Globally harmonized system Pictograms and Hazards:
corrosive to metals
corrosion
84
GHS (Globally harmonized system Pictograms and Hazards:
explosives
exploding bomb
85
GHS (Globally harmonized system Pictograms and Hazards:
self reactives
exploding bomb
86
GHS (Globally harmonized system Pictograms and Hazards:
organic peroxides
exploding bomb
87
GHS (Globally harmonized system Pictograms and Hazards:
oxidizers
flame over circle
88
GHS (Globally harmonized system Pictograms and Hazards:
aquatic toxicity
environment (non mandatory)
89
GHS (Globally harmonized system Pictograms and Hazards:
acute toxicity (fatal/toxic)
Skull and crossbones
90
Important part of the GHS and global chemical safety
Safety Data Sheet
91
includes information such as the properties of each
chemical; the physical, health, and environmental health
hazards; protective measures; and safety precautions
for handling, storing, and transporting the chemical.
SDS
92
Must be provided in a user-friendly, specific 16-section
format
SDS
93
How many sections are there in SDS?
16
94
What are the 16 sections of SDS?
Identification
Hazard Identification
Composition/Information on Ingredients
First-aid Measures
Firefighting Measures
Accidental Release Measures
Handling and Storage
Exposure Controls/Personal Protection
Physical and Chemical Properties
Stability and Reactivity
Toxicological Information
Ecological Information
Disposal Considerations (Non-mandatory)
Transport Information
Regulatory Information
Other Information
95
Among the most hazardous materials iin the clin chem lab
flammable/combustible chemicals
96
Classified according to flash point
flammable/combustible chemicals
97
flammable/combustible chemicals are classified accdg to what?
Flash point
98
