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1

This gland is not a specific pheromone producer gland:
- Rabbit’s face gland
- Gerbil’s umbilical gland
- Dog’s cicumanal gland
- Hamster’s hip gland

Rabbit’s face gland

2

Which is not a method for genetic quality control of lab animals?
a. Skin transplantation
b. Back gross breeding
c. SSLP (micro-satellite)
d. Biomedical markers

b. Back gross breeding

3

Drawback of preference tests:

Animals choose in the base of sort term conditions

4

Which measurement is used to express energy requirement of laboratory animals?

Metabolic body weight

5

Sentinels are:

Animals used to evaluate a group’s health status

6

Which method would you use to get 1mL of blood from a rat?

Cardiac puncutre

7

You cannot buy this type of diet from a commercial supplier:
a. Commercial pellet
b. Gel
c. Chemically defined diet
d. Liquid gel

c. Chemically defined diet

8

Not α2-agonist:
a. Detomidine
b. Tolmetine
c. Xylazine
d. Romofidine

b. Tolmetine

9

Not inherited disease of rat:
a. Hypo-acidosis
b. Deaf
c. Diabetes
d. Obesity

a. hypo-acidosis

10

The basic method of rodent phenotyping is to look for:

The chromosoma test

11

Not part of refinement of animal experimentation:
a. Genetic homogeneity
b. Health status
c. Alternatives to animal use
d. Proper defined diet

c. Alternatives to animal use

12

Which animals are covered by 63/2010 EEC directive?

Vertebrates and Cephalipodes

13

GLP not concerned about conditions under which laboratory:
a. Studies are planned
b. are build
c. research are performed
d. studies are recorded and reported

b. are build

14

Which species can die after oral penicillin treatment>

guinea pig

15

One of them is an important mechanism of nutrigenomics:

changing the signaling pathway

16

Acute toxicity test has an invasiveness category of __ pain:

Severe

17

Maintenance energy requirements of laboratory animals:

0.45MJ DE x W0.75

18

This disease is not a zoonosis:
a. Lymphatic choriomeningitis of mice
b. Salmonellosis
c. Ringtail
d. Listeriosis

c. Ringtail

19

The recommended nutrient allowances:

Ensure the reproduction and longevity of the animals

20

Cannot be stage of mouse genital and urinary syndrome:
a. Bacteriaemia
b. Ulceration
c. Inflammation
d. Mating overload

b. Ulceration

21

Quarantine period not determined by:
a. Health status of animals
b. Number of animals
c. Species
d. Purpose of use

b. Number of animals

22

Which species has a tied skin, so cannot grab it without causing pain to the animal?

Guinea pig

23

Not physical euthanasia method:
a. Cervical dislocation
b. T61
c. Decapitation
d. Maceration

d. Maceration

24

Which species is not a member of Rodentia genus?
a. Golden hamster
b. Marmoset
c. Guinea pig
d. Gerbil

b. Marmoset

25

OECD do not deal questions about:
a. Validation of in vitro methods
b. GLP
c. Co-ordination of toxicological databases
d. Validation of national laws

a. Validation of in vitro methods

26

Gerbil is a good model for:

epilepsy aorta

27

The animal cannot be examined with ultrasound:
a. Rabbit
b. Guinea pig
c. Golden hamster
d. Dog

Golden hamster

28

Not true for chronic mycoplasmosis of mice:
a. Zoonosis
b. Agent is Mycoplasma pulmonis
c. 12-18 day old puppies are most disposed
d. Can appear with Sendai virus

a. Zoonosis

29

The closes species to Syrian hamster in taxonomy is:

Gerbil

30

Good species to use for auditory research:

Chinchilla

31

Breeding technique which is not used to maintain an out-bred stock:
a. Cross-breeding
b. MAI
c. Random breeding
d. Brother-sister mating

d. Brother-sister mating

32

A contaminated breeding colony (line) should be: (?)
Euthanized
Put under negative pressure cabin
Treated by antibiotics
Individually marked

Treated by antibiotics?

33

In reproductive toxicology, not acceptable treatment: (?)
a. Used only for parents before mating
b. Between implantation and foetogenesis
c. Between foetogenesis and parturition
d. From parent before mating till separation from dam

?

34

It can be used for intra-dermal injection:

mice-ear

35

Pasteurella pneumotropica does not attack:
a. Prostate
b. Lung
c. Brain
d. Uterus

c. Brain

36

Environmental enrichment should not be done:
a. Just for human fun
b. For the benefit of the animals
c. With the sense of animals
d. With some unpredictability

a. Just for human fun

37

How would you restrain a gerbil?

Hand around the chest (think this is wrong)
o Neck and tail with two hands - I think this is more right

38

Who will decide on institute level if an animal experiment was necessary or not?

Ethical commitee (Animal Welfare Body)

39

It is not a zoonosis:
a. Hantaan virus disease
b. Salmonellosis
c. Sendai virus disease
d. Lymphocytic-ChorioMeningitis (LCM)

c. Sendai virus disease

40

This female embryo in a rat will achieve breeding maturity (M=Month):

1M

41

The basic method of rodent sexing is to look for:

Ano-genital distance

42

The recommended nutrient allowances:

Ensure optimum functioning of the animal

43

Maintenance energy requirements of laboratory animals and for extrapolation of dosage use:

W. 0.75

44

The main body of Laboratory Animal Science of the World:

CLAS

45

The closest species to Syrian hamster in taxonomy is:

Gerbil

46

What ‘Environmental Enrichment’ means in laboratory?

To give bedding material or toys to experimental animal if possible

47

Feeding rancid feed and/or vitamin E deficient feed may cause:

Dermatitis

48

Zebradanio is:

Omnivore

49

It is an alternative use of animals in experiments:

Database search and meta-analysis

50

Sub-cutaneous injury has a severity category of __ pain:

minor

51

Which 3 words called the 3 R s by Russel and Burch?

Reduction, Refinement, Replacement

52

It is not true about euthanasia: (?)
a.Can be used to get large amount of blood out of an animal
b. Must be performed by researcher or other trained person
c. Should be used if an animal would suffer in an experiment (this was marked as correct...)
d. Should be planned in experimental protocol

a.Can be used to get large amount of blood out of an animal

53

What does ‘knock out animal’ mean:

Transgenic animal

54

Which method would you use to get a drop of blood from mice?

Retro-orbital puncture

55

Which name indicates an outbred stock?

..lr:NMRI

56

You cannot use this method for euthanasia on rat:
a. Decapitation
b. Ketamine
c. Cervical dislocation with weight limit
d. CO2

b. Ketamine

57

What is the best equipment to maintain a transgenic colony in?

Base colony in different countries

58

Which species has only provoked (stimulated) ovulation?

Ferret

59

What does the expression ‘inbred strain’ mean:

Brother-sister mating throught at least 20 generations

60

Which species is the best in atherosclerosis research?

WHHL-rabbit
Notes - Gerbil

61

You can find a ‘sacculus rotundus or ampulla ilei’ in the:

Rabbit

62

This gland is a specific pheromone producer gland:

Hamster´s lateral gland

63

Which species has a gallbladder?

Golden hamster

64

What does SPF mean?

Specific pathogen free

65

How would you restrain a rat for per-oral dosing?

By grabbing the skin on neck and back

66

Which sentence is not true about ‘Environmental Enrichment’?
a. All possibilities should be done to improve animal’s well-being what the experiment allows
b. Always should be evaluated on cross-benefit base
c. Device in case that animal can control
d. A nest material is an example for that

c. Device in case that animal can control

67

Gnotobiotic (=Axenic) animals:

Germ free

68

What does rederivation mean?

Production of germ-free strain by embryo transfer

69

In the practice, it is not possible to produce germ-free colony from this species:
a. Rat
b. Golden hamster
c. Rabbit
d. Duck

d. Duck

70

Which would be the best feeding regime in an experiment?

Ad libitum

71

What is well-being?

State of physiological and psychological harmony of the animal with its internal and external environment

72

Which vein can be used for IV injection on ferret?

Jugular vein

73

How would you restrain a rabbit?

By grabbing as much skin as you can on the back

74

You cannot use this method for euthanasia on new-born, nest-living animals:
a. Cutting the neck
b. Cervical dislocation
c. CO2 inhalation
d. Electric stunning

b. Cervical dislocation

75

Which method would you use you get 10mL of blood from an adult rat?

Preparation of abdominal aorta

76

Which mouse strain is sensitive to Pneumocystis carini?

Swiss

77

Which is not necessary before transport of rodent?
a. Clinical examination
b. Fasting
c. Ear printing
d. Acclimatisation

b. Fasting

78

When this phenomenon disappears, the narcosis ends with death:

Cornea reflex

79

It is not true about euthanasia:
a. Should be cheap
b. Should achieve rapid unconciseness and death
c. Should be safe for user
d. Should be simple to administer

a. Should be cheap

80

One is not true about the co-isogenic strains:
a. It can only be maintained in a homozygous form
b. The new gene is the product of natural maturation
c. The name contains the strain/sub-strain and the symbol of the mutant gene
d. The co-isogenic strain only differs by only one gene from the original one

a. It can only be maintained in a homozygous form

81

Dog is not a good model for:
a. Tumour-cancer research
b. Leptospirosis
c. Epilepsy
d. Cardiovascular atherosclerosis

d. Cardiovascular atherosclerosis

82

Mini-pig has a live weight of:

20-45 kg

83

Experimental design cannot be:
a. Cross-over design
b. Cross-breeding
c. Latin square design
d. Factorial design

b. Cross-breeding

84

The basal circadian rhythm is:

Dependent from the gen expression of a protein

85

Melatonin is produced by:

Pineal gland

86

This species mostly used in viral respiratory research:

Ferrets

87

A chronic stress can cause this type of behaviour:

Apathy / Aggression

88

Well-being cannot be measured by: (more than one correct answer)
Clinical assessment
Physiological parameters
Grimace score
Reproductive state

??

89

Which environment is good for animals?

Classical music

90

Which tissue is the least sensitive to suffering?
a. dental pulp
b. brain tissue
c. skin
d. cornea

Brain tissue - think this is wrong

91

The naked mole rat has an extra=ordinary long life-span because of: (multiple choice)

Special hyaluronan which prevent cancer

92

The co-efficient on inbreeding (F): (multiple choice)

Means the fraction of the genes that get fixed in a homozygous state

93

It is inherited disease of some mouse strains:

Deafness

94

Which is a member of Pox viridae?

Ectomelia virus

95

Ringtail is caused by:

Too low relative humidity

96

Not part of refinement of animal experimentation:
a. Health status
b. Analgesia
c. Hair colour
d. Environmental enrichment

c. Hair colour

97

Give the meaning of the pyramid conception with examples.
Shortly describe the pyramid conception, with examples.

- Computer – Synthetize compounds
- Bacteria – Test for teratogenicity
- Cell culture – Eliminate harmful compounds
- Invertebrates
- Fish
- Mammals
- Non-human primates
- Humans (can be more steps in between)

98

Explain the epigenetics’ mechanism of action.

- Modifications e.g. Methylation, histone acetylation, which protect DNA and modify which genes are
expressed or silenced.
- Do not change the genome itself
- May be inherited
- Influenced by outside stimuli e.g. nutritional status

99

Explain the pain scoring system for Glires.
Describe the evaluation of the Glires pain categories.

- 0 points = None
- 1 point = Mild
- 2 points = Moderate
- 3 points = Severe
- Based on appearance, behaviour, pulse, appetite/drinking, cardiovascular, digestive, and nervous
systems.
- 0-4 = Normal
- 5-9 = Needs analgesia
- 10-14 = Moderate pain, possible need to terminate trial
- 15-20 = Not physiological, stop experiment!

100

Describe the naked mole rat as an animal model.

- Have a gene which conserves collagen.
- Do not age.
- Do not get tumours.
- Life-span of 20-30 years.
- Complex social system and hierarchy.
- Best model for aging and cancer studies.

101

The most used model for drug metabolism study for humans:

Macaco

102

May increase the mitochondria number:

Resveratrol

103

Carbohydrate ingestion:

Stimulate the fatty acid synthase gene expression

104

It can go through the uteral wall: (2 right answers)

Mouse mammy tumour
LCM

105

A chromorhnydachyrrhea:

Vitamin A deficiency

106

One of the statements is true:

Animals in an outbred stock are anisogenic

107

Always consider using F1 hybrid because:

Neither
Neither their biological response is more variable than the parental strains nor their biological repsonse is more uniform than the parental strains

108

The cephalopods:

Are under the protection of the 2010/63/EU Directive since 2013

109

One is true about the ferret:

We can prevent the constant estrus by using GnRH agonist implants

110

Pheromones are detected by:

VNO

111

You can find neoteny at:

Axolotl

112

In some cases the epigenetic changes may be inherited:

If the effect(s) acts during the germ-cell proliferation (‘time window’)

113

The role of __ is a fine regulation of the protein synthesis in the cell:

miRNA/ siRNA

114

One of them has no role in epigenomics:
a. Sulfurization
b. DNA-methylation
c. Histon acetylation
d. Change of base DNA

d. Change of base DNA

115

Zebrafish has a life-span of __year:

6-8 years
3-5 according to internet

116

One of them is an important mechanism of epigenomics:

Methyation of DNA

117

One of the following has no anti-cancer effect:
a. Iron
b. Resveratrol
c. CLA
d. Green tea

a. Iron

118

The role of PPAR is to:

Burn fats

119

Which species can die after oral antibiotic treatment?

Guinea pigs and rabbit

120

Laboratory animals – except the guinea pig – usually adjust their feed intake to satisfy their:

Energy requirement

121

Laboratory animals – except the guinea pig – usually adjust their feed intake to satisfy their:

Shows mating behaviour after HCG injection

122

The axolotl is not:
a. Carnivorous
b. Reaches adulthood after a complete metamorphosis
c. Capable of regenerating lost limbs completely
d. Has a ling lifespan (8-10 years)

Reaches adulthood after a complete metamorphosis

123

It is not a physical euthanasia method:
a. T61
b. Cervical dislocation
c. Cooling in refrigerator
d. Decapacitation

c. Cooling in refrigerator

124

The hearing range of rodents:

0.5/1 - 100/120 kHz

125

Which species has 1 pair teats?

Guinea pigs

126

Which species has a ‘peg’ (double incisors) teeth?

Rabbit

127

The two important words for animals concerning the pain feeling (Weiss-trail):

Predictability and controllability

128

Mouse pox is:

Caused by Ectomelia