Krebs Cycle

Question 1

Regulation of Pyruvate dehydrogenase complex

Answer 1

Inhibitors:
- ATP
- NADH
- Acetyl CoA
Activators:
- ADP
- NAD+
- CoA
- Pyruvate

Question 2

Regulation of Pyruvate Dehydrogenase with different ratios?

Answer 2

-High NADH/NAD Ratio
- high Acetyl Coa/CoA Ratio

-> inhibits PDC because enough NADH or Acetyl CoA is available

Question 3

Net equation of TCA cycle

Answer 3

Acetyl CoA + 3NAD+ + FAD + ADP + Pi + 2H₂O —> 2CO₂ + 3NADH + FADH₂ + ATP + 2H+ + CoA

Question 4

Key regulatory enzymes of tca cycle

Answer 4

1. citrate synthase
2. Isocitrate Dehydrogenase
3. Alfa-Ketoglutarate Dehydrogenase

Question 5

Anaplerotic pathways

Answer 5

1. Pyruvate + CO₂ -> Oxaloacetat
2. Amino Acids -> Glutamate -> Alfa-ketoglurate
3. Porphyrins -> Succinyl CoA