Proteins

Question 1

Cooperative binding of Oxygen by hemoglobin?

Answer 1

• it loads Oxygen in the lungs and unloads it in tissues where it is needed

Question 2

Role of 2,3-bisphosphatglycerate in the function of hemoglobin

Answer 2

• it reduces the affinity of hemoglobin to Oxygen
• enhances the unloading of oxygen in the tissues in hypoxic conditions

Question 3

Difference between fetal and maternal hemoglobin

Answer 3

• fetal hemoglobin has lower affinity to 2,3BPG than maternal hemoglobin
  -> fetal hem. binds oxygen more tightly

Question 4

Bohr effect

Answer 4

• decrease in pH reduces hemoglobins affinity for oxygen, making unloading of oxygen in tissues easier

Question 5

Carbon Monoxide compared to oxygen

Answer 5

• CM Binds to hemoglobin at oxigen binding Site 200 times more tightly
• binds to only 1 out of 4 oxygen binding Sites and prevents the hemoglobin from releasing oxygen

Question 6

Sickle cell disease

Answer 6

• some ß-globin chains have a mutation
• a change from Glutamat to Valin
• hemoglobin gets polymerased
• veins can get clocked -> ischamia