L29

Question 1

What drives microbiology?

Answer 1

Pathology

Question 2

Proaryotes make how much of the Earth’s biomass?

Answer 2

20 - 50%
*9.2 - 31.7 x 10^29

Question 3

How do we know that most organisms have not been cultured?

Answer 3

From what we have cultured using a unique fingerprint obtained from a 16S RNA mechanism, that is a protein synthesis process found in all organisms

Question 4

What does sequencing the 16S gene help us to do?

Answer 4

Help us to find the genera of the microbe

Question 5

How to microbes live on a grain of sand?

Answer 5

They have different metabolic processes, they oxidise the gases in the air

Question 6

What is a population

Answer 6

When a single microbe proliferates to form many individuals

Question 7

Community

Answer 7

Multiple populations interacting together

Question 8

Microbial ecology

Answer 8

The study of how microbes interact with each other and their environment

Question 9

What is enrichment culture

Answer 9

Enrichment culture - providing the temperature and
chemical conditions in the laboratory that encourage
the growth of specific groups of microbes.

Question 10

Beggiatoa

Answer 10

chemoautotroph, fixed CO2 from the atmosphere like a plant by oxidizing an inorganic molecule

Question 11

Mesocosm

Answer 11

an experimental system that simulates real-life conditions as closely as possible to study them in the lab

Question 12

Two goals from 1 process

Answer 12

*Harvesting energy
*12 of the building blocks to make things

Question 13

What do we all need?

Answer 13

An e- donor for energy, glucose –> oxidised to make CO2

Question 14

Are bacteria versatile in their reaction pathways

Answer 14

Yes as they can have different donors and acceptors

Question 15

What happens when H is oxidised

Answer 15

Releases protons and electrons

Question 16

Why does O2 in our body accept the H from the NADPH

Answer 16

It gets reduced to form CO2 as it has the most redox potential

Question 17

Where does NADPH do

Answer 17

Recycled to NADP+

Question 18

What is the energy harvested from the envir turn into

Answer 18

The local currency of the body, in humans its ATP

Question 19

Molecule that serves as intermediates to transfer
energy inside of cell

Answer 19

NADH/NADPH

Question 20

Conc and ratio of NADH/NADPH

Answer 20

Conc. stays the same but ratio changes as NADH/NADPH is recycled

Question 21

Two main areas:

Answer 21

Carbon source and Energy used

Question 22

Autotrophs use _____ while heterotrophs use ____

Answer 22

CO2, Organic Carbon

Question 23

Autotrophs

Answer 23

AUTOtrophs = Primary Producers
*Fix CO2
*Self sufficient, do not require carbon

Question 24

Heterotrophs

Answer 24

HETEROtrophs = Decomposers
*Need fixed carbon,
*cannot use CO2 directly
*CO2 is oxidised

Question 25

Chemotrophs

Answer 25

use chemical energy from either e- donors: * C compounds = organic *Non C compounds = inorganic

Question 26

Autotrophs

Answer 26

Uses solar E at diff wavelengths to syn ATP

Question 27

Chemoautotrophs

Answer 27

Fixes an inorganic molecule with Energy from the sun Makes own C source and then uses chem Energy to break bonds and make ATP

Question 28

Photoautotrophs

Answer 28

Fixes CO2 using E from the sun plants

Question 29

Chemoheterotrophs

Answer 29

Need Carbon molecules --> CO2 using chemical E obtained from organic/inorganic Our Energy and Carbon source are organic carbons

Question 30

Photoheterotrophs

Answer 30

Uses light to make E but their Carbon source is not made by them

Question 31

How do microbes in the same area exploit the same resource light

Answer 31

Light = common resource *Avoid competition by tuning their antenna to different wavelength i.e absorb diff wavelengths