L32-mitochondrial electron transport chain.

Question 1

what is mitochondrial electron transport chain?

Answer 1

final common pathway in aerobic cells that contain mitochondria by which electrons derived from various com pounds NADH+H+ and FADH2 are transferred to oxygen to form H2O this is associated with formation of energy in the form of energy.

Question 2

the oxidation of glucose and fatty acids donates electrons to specific co enzymes NADH, FADH2.
the reduced co enzymes can electrons to transport chains.
as the electrons pass down etc they lose much of their energy which used to pump protons into the pericisterna creating an electrostatic gradient across the inner membrane.

Question 3

protons will flow back into the mitochondrial matrix through ATP synthase and this will drive the formation of ATP form ADP and inorganic phosphate.
(Mitchels mosaic theory)
every ATP molecule requires 4 protons to be produced.

Question 4

what is oxidative phosphorylation?

Answer 4

its the coupling of electron transport chain with ATP synthase.

Question 5

what are the components of the electron transport chain?

Answer 5

# 4 protein complexes 3 of them spanning the inner mitochondrial membrane.
# 2 non protein molecules ubiquinone and cytochrome c
# the protein complexes have metals like iron and copper therefore have the ability to carry and lose electrons.

Question 6

complex I: NADH Dehydrogenase.

Answer 6

have many polypeptides
FMN coenzyme
seven iron sulfate centers
function: responsible for transferring electrons to the next member of etc (ubiquinone)
electrons are transferred from NADH+H+ to FMN coenzyme then to different to different iron sulfate center and then to ubiquinone.
4 protons pumped into the pericisterna.

Question 7

complex II succinate ubiquinone oxidoreductase

Answer 7

succinate dehydrogenase FAD coenzyme and 2 iron sulfate centers
it catalyzes the transfer of electrons form FADH2 released by oxidation of succinate to ubiquinone to form UQH2
no protons are pumped into the inner membrane space.

Question 8

Complex III: Ubiquinone ferricytochrome oxido reductase

Answer 8

one iron sulfate centers cytochrome b cytochrome c1

it transfers electrons from ubiquinone to cytochrome c