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Biochemistry > L6 > Flashcards

L6 Flashcards

1
Q

T/F All the dna is transcribed at one time

A

F, certain parts are transcribed at a time

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2
Q

Structural RNA include

A

Trna rrna

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3
Q

T/f: RNA can catalyze

A

Yes, ribozyme that cleaves rna molecules

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4
Q

What are the 4 rna species

A

28s 18s 5s 5.8s

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5
Q

% of
rRNA
mRNA
tRNA

A

80
3-5
15

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6
Q

Smallest rna

A

T

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7
Q

Most heterogenous

A

M

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8
Q

What is the main function of tRNA

A

mRNA Decoding during translation

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9
Q

Specific amino acid is attached to its 3’ end on the tRNA by what type of bond

A

Covalent

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10
Q

What are the requirements for transcription

A

1- chromatic remodeling
2- 3 types or RNA polymerase (pol l, pol ll, pol lll)
3-transcription factors (TF)

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11
Q

REGULATORY PROTEINS that INITIATE and REGULATE transcription

A

TFs

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12
Q

TFs job

A

Regulate and initiate transcription
Tells the primer where to bind

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13
Q

Examples of regulatory proteins

A

TFs

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14
Q

Page 5 second slide read it

A

Okay

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15
Q

What are the types RNA polymerases and their function

A

A) nuclear

Pol l:
pre- rRNA
precursor for 28s 18s 5.8s in the nucleolus

Pol ll:
mRNA
miRNA snRNA snoRNA

pol lll:
tRNA
5s
snRNA snoRNA
——————
B) Mitochondrial RNA pol:
Synthesis of all types of mtRNAs

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16
Q

TATA box are ___ nt upstream
While CAAT box is __

A

25
70-80

17
Q

TATA and CAAT are ___

A

Dna elements = promoter region

18
Q

Why is the promoter region not translated ?

A

Because it is -ve nt

19
Q

Who recognizes dna elements

A

General TFs

20
Q

What are the 3 example of TFs and th what dna elements for they bind

A

CTF: CAAT BOX
TFllD: TATA BOX
SP1: GC BOX

21
Q

What are the functions of TFs

A

-recruits RNA polymerase
-assembly of the transcription factors

22
Q

T/F: each transcription factor has a certain DNA elements

A

T

23
Q

What does alpha-amanitin

A

Forms a tight complex with RNA pol ll and inactivated it —> inhibits mrna synthesis

24
Q

recruits RNA polymerase

A

TFs

25
Q

assembly of the transcription factors

A

TFs

Biochemistry (9 decks)

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