L6 Flashcards
(25 cards)
T/F All the dna is transcribed at one time
F, certain parts are transcribed at a time
Structural RNA include
Trna rrna
T/f: RNA can catalyze
Yes, ribozyme that cleaves rna molecules
What are the 4 rna species
28s 18s 5s 5.8s
% of
rRNA
mRNA
tRNA
80
3-5
15
Smallest rna
T
Most heterogenous
M
What is the main function of tRNA
mRNA Decoding during translation
Specific amino acid is attached to its 3’ end on the tRNA by what type of bond
Covalent
What are the requirements for transcription
1- chromatic remodeling
2- 3 types or RNA polymerase (pol l, pol ll, pol lll)
3-transcription factors (TF)
REGULATORY PROTEINS that INITIATE and REGULATE transcription
TFs
TFs job
Regulate and initiate transcription
Tells the primer where to bind
Examples of regulatory proteins
TFs
Page 5 second slide read it
Okay
What are the types RNA polymerases and their function
A) nuclear
Pol l:
pre- rRNA
precursor for 28s 18s 5.8s in the nucleolus
Pol ll:
mRNA
miRNA snRNA snoRNA
pol lll:
tRNA
5s
snRNA snoRNA
——————
B) Mitochondrial RNA pol:
Synthesis of all types of mtRNAs
TATA box are ___ nt upstream
While CAAT box is __
25
70-80
TATA and CAAT are ___
Dna elements = promoter region
Why is the promoter region not translated ?
Because it is -ve nt
Who recognizes dna elements
General TFs
What are the 3 example of TFs and th what dna elements for they bind
CTF: CAAT BOX
TFllD: TATA BOX
SP1: GC BOX
What are the functions of TFs
-recruits RNA polymerase
-assembly of the transcription factors
T/F: each transcription factor has a certain DNA elements
T
What does alpha-amanitin
Forms a tight complex with RNA pol ll and inactivated it —> inhibits mrna synthesis
recruits RNA polymerase
TFs
