Lab 1 Flashcards
(46 cards)
Laboratory safety
Strict adherence to prescribed rules for personal safety and environmental safety
Personal safety
To avoid laboratory accidents
Environmental safety
To prevent contamination of experimental procedures by microorganisms from exogenous sources
Virulence
Is the ability of a microorganism to infect or damage a host
Virulence of the microorganisms used in our laboratory greatly diminished ⬇️
Because of their long term maintenance on artificial media
Pathogens
Organisms capable of producing disease
Results , Use _________________ in our laboratory work
Aseptic techniques
Aseptic techniques
Practices and procedures to prevent microbial contamination
??
Use aseptic techniques
Because most microbiological laboratory procedures require the use of living organisms
Environmental safety rules
- Place coats , books and other paraphernalia in specified locations ✅ ….never on bench tops ❎
- Keep doors and windows closed during the laboratory session to prevent contamination from air currents
- wipe bench tops with a disinfectant solution
- don’t palce contamination instruments , sush as inoculating loop ,needles , and pipettes , on bench tops.
- Place all culture and materials in the disposal area as designated by the instructor.
- Rapid and efficient manipulation of fungal cultures is required to prevent the dissemination of their reproductive spores in the laboratory environment
Loops and needles should be sterilized by ______________, and pipettes should be disposed of in _____________.
Incineration / designated receptacles
To prevent accidental injury and infection and personal safety rules
- Wash your hands with liquid detergent , rinse with 95% ethyl alcohol, and dry them with paper towels upon entering and prior to leaving the laboratory
- always use the appropriate safety equipment
- Wear a paper cap or tie back long hair
- Wear closed shoes at all times
- Never apply cosmetics or insert contact lenses in the laboratory
- Don’t smoke , eat and drink.
- Carry cultures in a test tube rack when moving around the laboratory
- Never remove media , equipment or especially , microbial cultures from the laboratory
- Immediately cover spilled cultures or broken culture tubes with paper towels and then saturate them with disinfectant solution. After 15 minutes of reaction time, remove the towels and dispose of them in a manner indicated by the instructor
- Report accidental cuts or burns to the instructor immediately
- Never pipette by mouth any broth cultures or chemical reagents. Pipetting is to be carried out with the aid of a mechanical pipetting device only
- Don’t lick labels. Use only self stick labels for the identification of experimental cultures
- speak quietly and avoid unnecessary movement around the laboratory
appropriate safety equipment
- Lab Coat or apron
- Gloves
- Mask and safety goggles 🥽
Lab Coat protect
Clothing from contamination or accidental discoloration by staining solutions
Gloves shield
Your hands from contamination by microorganisms.
Prevent the hands from coming in direct contact with stains and other reagents
Mask and safety goggles 🥽
To prevent materials from coming in contact with your eyes
Mask and safety goggles 🥽
To prevent materials from coming in contact with your eyes
??
Wear a paper cap or tie back long hair
To minimize it’s exposure to open flames
??
Carry culture in a test tube rack when moving around the laboratory
To Prevent accidents and to avoid contamination of yourself and the environment.
??
avoid unnecessary movement around the laboratory
To prevent distractions that may cause accidents
??
The following specific precautions must be observed when handling body fluids of unknown origin
due to the possible transmission of the HIV and hepatitis B viruses in these test specimens
The following specific precautions must be observed when handling body fluids of unknown origin
- Wear disposable gloves during the manipulation of test materials such as blood , serum , and other body fluids .
- Immediately wash hands if contact with any of these fluids occurs and also upon removal of the gloves .
- Wear masks , safety goggles , and laboratory coats if an aerosol might be formed or splattering of these fluids is likely to occur .
- Decontaminate spilled body fluids with a 1:10 dilution of household bleach , covered with paper toweling , and allowed react for 10 minutes before removal .
- Place test specimens and supplies in contact with these fluids into a container of disinfectant prior to autoclaving .
Laboratory Protocol
- Microscopic slides must be wiped with dry lens paper to remove dust and finger marks prior to their use
- Petri dishes must be inverted during incubation to prevent water condensation from dropping onto the surface of the culture medium
Pipettes must be sterilized by
autoclaving
