Lab#1 Flashcards

1
Q

Monomer

A

The molecule/subunit which acts as a building block for polymers.

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2
Q

Polymer

A

A long molecule consisting of many similar or identical monomers linked together by covalent bonds.

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3
Q

PCR

A

Polymerase Chain Reaction

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4
Q

DNA

A

Deoxyribonucleic Acid

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5
Q

in vitro

A

Performing a given procedure in a controlled environment OUTSIDE of a living organism .
(Partial or dead)

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6
Q

in vivo

A

Experimentation using a whole, living organism.

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7
Q

Locus

A

A specific place along the length of a chromosome where a given gene is located.

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8
Q

Allele

A

Any of the alternative versions of a gene that may produce distinguishable phenotypic effects.

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9
Q

DNA is the _____ of all living things

A

Genetic Material

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10
Q

DNA contains all the necessary info. to direct the _________ of an organism

A

Growth and Maintenance

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11
Q

DNA directs all cell _____ and determines all of an organism’s ______

A

Activities; Traits

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12
Q

DNA typically stored in….

A

Tightly coiled structures called chromosomes

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13
Q

Tiny proteins called ____ hold the network together. (In DNA)

A

Histones

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14
Q

Remove histones….?

A

DNA unravels like a long chain

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15
Q

Each link in DNA is called….?

A

Nucleotide

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16
Q

Many nucleotides in a chain….

A

Poly(many)nucleotide

17
Q

Sides of the DNA helix are….?

A

The sugar phosphate backbone

18
Q

The rungs of the ladder are…?

A

The base pairs held together by hydrogen bonds

19
Q

Where are the instructions for protein building?

A

Somewhere on your DNA

20
Q

The four bases are?

A

Guanine, Thymine, Adenine, Cytosine

21
Q

Each base is shaped _____ in comparison with the other three.

A

Differently

22
Q

In base pairing….who pairs with who?

A

Adenine-Thymine (dbl bond)

Guanine-Cytosine (trpl bond)

23
Q

Names of each end of a polynucleotide.

A

3’,5’ (know which end is which!)

24
Q

Why does DNA need to replicate?

A

Every cell in your body needs a complete copy of your entire DNA library.

25
original molecule unzipped by
original molecule unzipped by helicase
26
New nucleotides move in and elongate the daughter strand w
DNA polymerases
27
DNA polymerase adds nucleotides only to the ____end of a growing daughter strand
3'
28
A growing daughter strand elongates only in the ____ direction
5 -> 3
29
Individual with only one band....
Individual homozygous
30
3 bands
Allelic Ladder