lab 14 Flashcards by angi nikoula

Q

What chemical does Amylase test detect?

A

presence/absence of starch

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Q

What enzyme(s) is/are involved in this test?

A

Amylase

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Q

What substrate(s) is/are involved in this test?

A

Amylose (starch)

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Q

What is the indicator/reagent used in this test?

A

Iodine

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Q

What is the appearance of a positive result?

A

clear around the growth

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Q

What is the appearance of a negative result?

A

Blue / Black around bacterial growth

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Q

What chemical does the Urease test detect?

A

Ammonia- change in PH

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Q

What enzyme(s) is/are involved in this test?

A

Urease

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Q

What substrate(s) is/are involved in this test?

A

Urea

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Q

What is the indicator/reagent used in this test?

A

Phenol Red

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Q

What is the appearance of a positive result?

A

Hot Pink

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Q

What is the appearance of a negative result?

A

orange or yellow

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Q

What chemical does the Catalase test detect?

A

Oxygen

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Q

What enzyme(s) is/are involved in this test?

A

Catalase

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Q

What substrate(s) is/are involved in this test?

A

Hydrogen peroxide

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Q

What is the indicator/reagent used in this test?

A

Hydrogen peroxide

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Q

What is the appearance of a positive result?

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A

Bubbles

Q

What is the appearance of a negative result?

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A

No Bubbles

Q

What chemical does the SIM Sulfur test detect?

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A

Hydrogen Sulfate

Q

What enzyme(s) is/are involved in this test?

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A

Cysteine desulfurase and thiosulfate reductase

Q

What substrate(s) is/are involved in this test?

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A

Cysteine/sodium Thiosulfate

Q

What is the indicator/reagent used in this test?

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A

Ferrous Sulfate

Q

What is the appearance of a positive result?

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A

Black precipitate

Q

What is the appearance of a negative result?

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A

No Black precipitate

What chemical does SIM Indloe test detect?

Indole

What enzyme(s) is/are involved in this test?

Tryptophanase

What substrate(s) is/are involved in this test?

Tryptophan

What is the indicator/reagent used in this test?

Kovac's reagent

What is the appearance of a positive result?

Red ring

What is the appearance of a negative result?

No change

What chemical does SIM Motility test detect?

movement of bacteria

What enzyme(s) is/are involved in this test?

N/A

What substrate(s) is/are involved in this test?

N/A

What is the indicator/reagent used in this test?

N/A

What is the appearance of a positive result?

Growth

What is the appearance of a negative result?

Not cloudy throughout media / Growth only along stab line

What chemical does Citrate test detect?

Ammonia (basic pH)

What enzyme(s) is/are involved in this test?

Bromthymol blue

What substrate(s) is/are involved in this test?

Citrate

What is the indicator/reagent used in this test?

Citrate lyase & Citrate permease

What is the appearance of a positive result?

Royal Blue

What is the appearance of a negative result?

Forest Green