lab 8 Flashcards
(16 cards)
What is the primary dye used in the endospore stain?
Malachite green
What is the decolorizer used in the endospore stain?
Distilled water
What is the secondary dye used in the endospore stain?
Safranin
What is the final color of the endospore in the endospore stain?
Green
What is the final color of the vegetative cell in the endospore stain?
Red or pink
What is the primary dye used in the capsule stain?
Congo Red
What is the decolorizer used in the capsule stain?
None.
What is the secondary dye used in the capsule stain?
Flood the slide with Maneval’s
What is the final color of the background in the capsule stain?
Blue
What is the final color of the cell in the capsule stain?
Hot pink
What is the final color of the capsule in the capsule stain?
clear halo around the microorganism
What dye is used as the primary dye for the Acid Fast stain?
Carbol Fuchsin
What is the decolorizer used for the Acid Fast stain?
Acid-Alcohol (3% HCI)
What dye is used as the secondary dye for the Acid Fast stain?
Methylene Blue
What is the final color of Acid Fast bacteria?
Hot pink/ Red
What is the final color of Non-Acid Fast bacteria?
blue
