Lab 2: Cytoskeleton: Cell Motility and Subcellular movement

Question 1

define pure culture

Answer 1

an isolated group of genetically identical cells

Question 2

define colony

Answer 2

small mass of genetically identical cells

Question 3

define medium

Answer 3

is a solution or solid that contains nutrients that cells can use to grow and divide

Question 4

define lawn

Answer 4

lawn of bacteria, a dense, even layer of bacteria on the play with clearings where the antibiotics kill the cell

Question 5

microtubules

Answer 5

cilia and flagella are made from this and they are eukaryotic motile structures composed of microtubules

Question 6

microfilaments

Answer 6

causes pseudopodia, movement of the cell by assembling and disassembling networks of microfilaments

Question 7

what increases contrast

Answer 7

stains
decrease light
use special microscopes: dark field, phase, and fluorescence

Question 8

what increases resolution

Answer 8

*smaller resolution values the better
- small wavelength
increase numerical aperture of the objective lens
add immersion oil

Question 9

summary of lab:
-observation of intracellular movemnts, motility, and feeding behaviours
-observation of a mixed culture isolated from spinach