Lab 4: Cell Phone Biology and Antibiotic Resistance

Question 1

Transmittance

Answer 1

trasmitted light to incident light, amount of light that passes through

Question 2

Absorbance

Answer 2

no units, measures the amount of light that is absorbed, will be a linear function of solute concentration

Question 3

D = volume of original solution/volume of original +volume of solvent

eg. add 0.5ml of the original solution to 4.5ml of buffer

Answer 3

D=0.1

Question 4

what can you do if the absorbance doesn’t fall within the range of the standard curve?

Answer 4

redo the dilution with more solvent (buffer/ mainly water) and less original sample (so that it has a smaller ‘D’)

Question 5

Cu = Cd/D

Question 6

would we see the same culture growth on different parts of our body?

Answer 6

no, different areas of our skin have distinct compositions of prokaryotes and fungi

Question 7

summary of the lab:
creating a standard curve
examining phone imprints
examining swabbed plates for antibiotic resistant bacteria