Lab 3 Flashcards
(15 cards)
Endospores are common in
Bacillusm and clostridium
serial dilution is used to
determine the original population density of a bacterial culture
Whats the primary stain in endospore staining
Malachite green
Spore coat is made of
keratin and dipicolinic acid (high in calcium)
The primary stain for spore staining
Malachite green
The secondary stain is
Safranin
Age of culture used
old culutre
First step of spore stain
air dried, heat fixed
What should be seen in the positiv control
green endospores within the red vegetative part
What should be seen in the negative control
all red rods
Color of acid fast vs. non acid fast
acid fast: pink
non acid fast: blue
Serological pipette
graduation goes all the way down -> blow out pipette/ to deliver
Mohr pipettes
non blow out, graduation stops
Final dilution factor =
Dilution factor x volume plated
