lab 4-6 Flashcards
1
Q
Application of EMB?
A
Used for isolation of fecal coliforms
2
Q
What kind of medium is EMB?
A
(DUCS) Differential Undefined Complex Selective
3
Q
What makes the EMB selective?
A
Gram -. EMB inhibits the growth of gram positive.
4
Q
What makes EMB differential?
A
Lactose fermenters vs non lactose fermenters
5
Q
What does EMB contain?
A
(GEML)
Gelatin: yields carbon and nitrogen
Eosin Y & Methylene Blue
Lactose: ferments acid-end products by coliforms
6
Q
Purpose of the dye’s?
A
- They inhibit the growth of most gram positive organisms.
2. They react with vigorous lactose fermenters who acid-end products turn the growth dark purple or black.
7
Q
What makes EMB undefined and complex?
A
Digest of gelatin present
8
Q
Application of EMB?
A
Used for isolation of fecal coliforms
