Lab 4: Microscopy And The Cell Flashcards
(23 cards)
How long has life existed on Earth??
At least 3.5 billion years.
When was the microscope invented??
1590
Prokaryotic cell??
Most primitive cell type. Lack membrane bound nucleus and organelles. Later evolved into eukaryotic cells.
Eukaryotic Cells??
Do have a nucleus and membrane-bound organelles.
List the 3 domains of life on Earth??
1: eukarya
2: archaea
3: bacteria.
(2-3 are prokaryotic cells)
What microscopes were utilized during the experiment?
1: Compound Microscope
2: Dissecting Microscope
Define Compound Microscope??
Microscope that uses at least 2 lenses in cooperation to magnify an object.
Describe components of a COMPOUND MICROSCOPE??
1: HEAD
-Ocular Sense (10x magnification)
-Camera Attachment Tube
-Revolving Nosepiece
- 4 Objective Lenses
A) 4x scanning lens
B) 10x objective lens
C) 40x high-dry lens
D) 100x
2: ARM
-Stage
A) mechanical stage
B) condenser
C) iris diaphragm
D) condenser adjust.
E)mech. stage adjust
-Light switch
-light intensity knob
3: BASE
-sub stage light
-coarse focus knob
A) fine focus knob
Define DISSECTING SCOPE??
(STEREOSCOPE) used to show detail, but object is too large/thick for Compound Scope.
Define ARCHAEA??
Extremophiles prokaryotes.
Thermus Aquaticus
Activity 1: Archaea. It’s Taq enzymes are used to recreate millions of DNA without cells due to the enzymes not denaturing. Purple dots.
Anabaena.
Activity 2: Photosynthetic Bacteria. Green rings.
Physarum??
Activity 3: Slime mold of Protists.
Paramecium??
Single-celled animal-like Protists.
Depression Slide??
Slide with a well in it.
List Domain Eukarya Kingdoms??
1: Protista
2: Plantae
3: Fungi
4: Animalia
List members of EUKARYA??
1: land plants
2: algae
3: animals
4: fungi
5: ciliates
6: diatoms
7: euglena
List members of ARCHAEA KINGDOM??
1: halophiles
2: thermophiles
3: sulfolobus
4: methanobacterium
List members of KINGDOM BACTERIA??
1: mitochondrion
2: Cyanobacteria
3: chlamydia
4: spirochetes
5: green sulfur/nonsulfur bacteria.
Procedure for procuring and using Microscopes??
1: retrieve with 2 hands.
2: wipe dust off all glass with LENS PAPER
3: lower stage all the way down.
4: objective lens at lowest setting.
5: place slide on stage and secure.
6: adjust using mech. stage adjustor
7: rotate Course Focus knob.
Difference of animal/plant cells??
1: chloroplast
2: cell wall
3: vacuole
Endosymbiotic Theory??
States that eukaryotic organelles evolved from aerobic prokaryotes.
Describe components of a DISSECTING MICROSCOPE??
1: OCULARS
2: OBJECTIVE
-incident light
-focusing knob
3: BASE
-on/off switch
-stage
A) transmitted light
