LAB

Question 1

Toluidine Blue

Answer 1

Stains cellulosic Walls purple and lignified walls light blue. Is a good general purpose stain.

Question 2

Aniline Blue

Answer 2

Stains nuclei, cytoplasm and callose blue

Question 3

phlorogucinol/HCL

Answer 3

PREPARE A 1:1 SOLUTION IN A SMALL PETRI DISH AND FLOAT SECTIONS FOR 2-5 MINUTES FOR STAINING. stains lignified walls red.

Question 4

IKI

Answer 4

Stains starch grains dark blue; cellulose walls brown; cork, cutin and callose yellow.

Question 5

Ruthenium Red

Answer 5

Stains pectins red.

Question 6

Clearings

Answer 6

NaOH is used to remove the cellular contents to make clearings. After treatment only the cell walls remain. Clearings are commonly used to examine patterns of cell division, epidermal structures and patterns of vascular tissue.

Question 7

Macerations

Answer 7

Tissue treated with acid or base which dissolves the middle lamella (glue between cell walls) and causes the individual cells to come apart allowing them to be viewed individually.

Question 8

Root System

Answer 8

Tap root, lateral roots, root hairs

Question 9

Shoot system

Answer 9

cotyledons, hypocotyl, stem (nodes and internodes); foliage leaves (base, petiole and lamina); Leaf axil; axillary buds; shoot apex; leaf primordia (youngest leaves)

Question 10

Helianthus

Answer 10

Sunflower