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AP Biology > Lab 6 > Flashcards

Lab 6 Flashcards

1
Q

What does a respirometer calculate

A

Rate of oxygen consumption or cellular respiration

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2
Q

What type of seed is used

A

Pea seeds

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3
Q

Seed germination

A

Whether a seed has broken dormancy

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4
Q

What is reviewed and applied for this lab

A

Ideal gas law

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5
Q

What are individual cells responsible for

A

Energy exchanges necessary to sustain ordered structure

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6
Q

How do cells do necessary energy exchanges

A

Breaking down nutrient molecules to generate ATP

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7
Q

What does cell respiration require

A

Nutrient molecules and oxygen

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8
Q

What is cell respiration

A

Breaking down nutrient molecules to generate ATP to fuel cell processes

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9
Q

What are products of cell respiration

A

Carbon dioxide and water

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10
Q

Equation for cell respiration

A

C6H12O6 + 6O2 –> 6CO2 + 6H2O

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11
Q

Ways to measure rate of cell respiration

A

Monitoring changes in temperature

Measure oxygen consumption or carbon dioxide production

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12
Q

Why can temperature be used

A

Respiration is Exergonic and produces heat

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13
Q

What is a respirometer

A

Devices that measure gas volume changes

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14
Q

What is needed to use a respirometer

A

Ideal gas law

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15
Q

Ideal gas law

A

Describes relationship between temperature, pressure, and volume
PV=nrT

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16
Q

What gases are changing in volume during cell respiration

A

Oxygen and carbon dioxide

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17
Q

What is happening to oxygen

A

Consumed by respiring cells

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18
Q

What is happening to carbon dioxide

A

Diffusing out

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19
Q

How can the respirometer deal with two gases

A

Potassium hydroxide which absorbs carbon dioxide

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20
Q

KOH formula

A

CO2 + 2KOH –> K2CO3 + H2O

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21
Q

What is potassium carbonate

A

A solid precipitate

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22
Q

What happens to carbon dioxide when potassium hydroxide is added

A

Turns into a solid, so respirometer will only measure consumption of of oxygen

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23
Q

What respirometers will be used and how

A

2 sets of 3, submerged in water
Each set at a different temperature
One with germinated seeds, one with non-germinating seeds and plastic beads, and one with beads
Also contain cotton saturated with KOH

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24
Q

Why the beads

A

To ensure each respirometer is uniform in volume

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25
Why cotton
Carbon dioxide will be absorbed
26
What will happen in pan of water
Water will flow from high pressure to low pressure Oxygen used up by seeds, gas pressure inside respirometer will decrease, water will flow into the pipette down pressure gradient
27
What do pictures look like
``` Pipet tip Seeds on top Beads Dry cotton Absorbent cotton with KOH Weights underneath ```
28
How many of each type of seed
50
29
How will the beads be
In a container
30
Ho many stoppers
6
31
What will be attached to stoppers
1 mL graduated pipettes
32
How many paper towels
6
33
How many water bath containers
2
34
What type of graduated cylinder
100 mL
35
How many thermometers
2
36
What other materials
``` Absorbent cotton No absorbent cotton Sharpie Ice Masking tape Cell phone timer Bucket of water Scissors Plastic trash bucket ```
37
What will be in the dropper bottle
15% KOH
38
What about respirometer tops
Be careful They're rubber with pipette sticking out Will lose seal
39
Step 1
Label paper towels 1a, 2a, 3a, 1b, 2b, 3b
40
What is step 2
Label respirometer a with sharpie, same as paper towels
41
Step three
Fill grad cylinder with 50 mL of water Put 25 germinating seeds in the cylinder Record volume in Data Table 1a Subtract 50 mL to determine total volume of seeds and record same place Pour out water into trash bucket and put seeds on paper towel 1a
42
What is step 4-5
Same as step 3, but after you do 25 dry seeds, count in plastic beads until volume matches Record same place Put mixture on 2a
43
Step 6
Same as last ones, but add beads till get same volume Data table 1a Beads on paper towel 3a
44
What is step 7
Do it for the b respirometer said and record in 1b
45
What are the lines at in the pipette
Hundredths
46
Step after weighing
Put absorbent cotton to bottom of weighted vials, push down with wooden stick or stirring rod Saturate with 4 drops of 15% KOH
47
About KOH
Caustic
48
After absorbent cotton
No absorbent cotton, push to bottom
49
Why non absorbent
Peas don't touch poisonous KOH
50
After cotton
Add peas and beads Put in stoppers Should be tight and not wiggle much
51
Where does part 2 start
After filling vials
52
Part 2 step 1
Will baths with tap water 1 inch from top Room temp, and cold temp by adding ice til 10 degrees Celsius and 25 Celsius Continue adding ice, ask Mallett
53
Second step part 2
Masking tape across narrow width of bath, 2/3 way from 1 end | Wither notebook paper in bottom of tub to read pipettes easier
54
Step three part 2
Place thermometers on floors of baths, take temps record 1C
55
Step 4 part 2
Put a respirometers in room temp water bath so pipette rest on masking tape Same with b, but put them in cold
56
What to do after placing respirometeso
Time for 7 minutes - equilibration period to be same temp as water Don't hit bath or table at any point
57
Start time then
Mrs. Mallet show you how to put in food coloring
58
Where will lab need to be fast
After food coloring
59
Fast part
At end of 7 minutes, put tubes entirely in baths Cut tape ends so respirometers go in gently Some water gets in pipettes, but influx should stop quickly, if it continues check for leaks and call for Mallett
60
Second step fast
Make sure you can read scale Air bubble should extend from main chamber up tube of pipette Record water level onto the data table at time interval 0 Record position of water in each pipette 5, 10, 15, 20 min data table 2
61
Clean up
Due respirometers onto paper towel and rinse beads put them back in container from Mallett so they dry Throw out peas Scissors to pull out cotton and throw away Slide water tubs toward sink and dump water - careful Sham wow dry water from pans Rinse pipettes with distilled water Once water is clear,s hake pipette over sham wow to remove as much water as possible and return pipette where instructed, don't break it or its seal
62
Two questions
How temp and seed germination affect rate of respiration

AP Biology (4 decks)

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