Lab 8

Question 1

What is the function of a restriction enzyme?

Answer 1

Recognize and cut double stranded DNA sequences at a sequence-specific site

Question 2

How can restriction enzymes can be used in cloning?

Answer 2

Able to cut the DNA sequences and pastes a new one with ligase

Question 3

How can restriction enzymes can be used in diagnostic digests?

Answer 3

Restriction enzymes are able to cut the pieces needed the the diagnostic

Question 4

What are the 5 components of a PCR

Answer 4

1. Template (dsDNA)
2. Primers (orientation)
3. dNTPs
4. Taq polymerase
5. Buffer

Question 5

Normal PCR’s are not…….

Answer 5

Quantitative